湖南农业大学学报（自然科学版）2017，Vol.43Issue(4)：405-410,6.DOI:10.13331/j.cnki.jhau.2017.04.011
茶叶提取物EGCG和GCG及ECG对B16细胞内黑色素生成的抑制作用
Inhibitory effects of tea extracts EGCG,GCG and ECG on the melanogenesis in melanoma cell B16
摘要
Abstract
Inhibitory effects of tea extracts EGCG, GCG and ECG on melanoma cell B16 from mouse were studied in this paper. Cells were treated with different concentrations of GCG, ECG, EGCG and Arbutin, and their morphology was observed in an inverted microscope. The proliferation rate of B16 was determined using MTT method, tyrosinase activity was detected by using L-DOPA as substrate, and NaOH-lysis approach was employed to measure the melanin synthesis. The results showed that EGCG, ECG, GCG could significantly inhibit the melanogenesis and tyrosinase activity in cells B16 with a dose-dependent manner. The proliferation rate would be lower than 50% when the concentration was increased to 60 μg/mL. Compared with the control group, tyrosinase activities affected by the three substances were reduced by 26.67%, 27.27% and 32.71%, respectively, the melanin inhibition rates were all above 30%. In conclusion, tea extracts could efficiently inhibit melanogenesis. The inhibitory effect of GCG was best, followed by ECG, EGCG, and arbutin in turn.关键词
茶叶提取物/表没食子儿茶素没食子酸酯/没食子儿茶素没食子酸酯/表儿茶素没食子酸酯/B16黑色素瘤细胞/黑色素生成/酪氨酸酶Key words
tea extracts/epigallocatechin gallate (EGCG)/galllocatechingallate(GCG)/epicatechingallate(ECG)/B16分类
农业科技引用本文复制引用
张向娜,林勇,黄建安,刘仲华,梁丹丹..茶叶提取物EGCG和GCG及ECG对B16细胞内黑色素生成的抑制作用[J].湖南农业大学学报（自然科学版）,2017,43(4):405-410,6.基金项目
国家自然科学基金项目(31100502) （31100502）
国家现代农业茶叶产业技术体系项目(CARS-23) （CARS-23）
