海洋科学2017，Vol.41Issue(4)：1-9,9.DOI:10.11759/hykx20160528002
紫外辐射对孔石莼抗氧化酶活性及其同工酶表达的影响
Effect of UV radiation on the activities of antioxidant enzymes and their isoforms in Ulva pertusa(Chlorophyta)
摘要
Abstract
Themarine ecosystem inevitably undergoes stress because of exposure to enhanced UV-B radiations. The response of the antioxidant defense system, including that of SOD, CAT, POX, APX, GR, and GPX, of the intertidal macroalgae Ulva pertusa to different doses of UV-B radiation and the first four isoenzyme patterns were investi-gated. SOD, CAT, and POX rapidly responded to UV-B radiation. The response significantly increased during the initial stage of UV-B treatments. The APX activity remained at a more stable level throughout the exposure. The responses of GR and GPX to UV-B stress were observed later, and an activity peak appeared on day 6. The enzy-matic assay revealed four distinct bands of SOD, and SODⅠ and SODⅡ activities increased following exposure to UV-B radiation. The activity of POXⅡ isoenzyme slightly increased following the exposure to UV-B radiation. In addition, new bands of CATⅠ and CATⅡ were detected in response to UV treatments. Only one APX band was detected, and there was no significant difference with respect to Ck. The concentrations of antioxidants such as AsA and GSH were similar to those in the control during the early-to-medium term. SGR reduced following expo-sure to all three doses, including 1.6, 4.8, and 9.6 kJ/(m2·d), and significantly decreased in the latter two treatments. H2O2 and TBARS concentrations significantly increased compared with those in the control. These data suggested that the protection mechanisms of the antioxidant defense system are partly inducible by UV-B to prevent damage and that the enzyme activities change in response to UV-B radiation in a time- and dose-dependent manner.关键词
UV-B辐射/孔石莼(Ulvapertusa)/抗氧化体系/同工酶Key words
UV-B radiation/Ulva pertusa/antioxidant system/isoenzyme分类
资源环境引用本文复制引用
李丽霞,王秀静,赵吉强..紫外辐射对孔石莼抗氧化酶活性及其同工酶表达的影响[J].海洋科学,2017,41(4):1-9,9.基金项目
国家自然科学基金青年项目(31300326) [National Natural Science Foundation of China , No.31300326] （31300326）
