海洋科学2017，Vol.41Issue(4)：17-23,7.DOI:10.11759//hykx20160801001
施氏鲟Asnanos1基因序列分析及其在雌雄不同组织中表达
Sequence and differential expression analyses of theAsnanos1 gene in different tissues of male and female Acipenser schrenckii
摘要
Abstract
The full-length cDNA of the Asnanos1 gene was obtained from the gonadal transcriptome of Acipenser schrenckii. The sequence was confirmed by polymerase chain reaction (PCR) and analyzed with a bioinformatics tool. The expression levels of Asnanos1 mRNA in gonad, heart, gill, brain, kidney, and spleen were examined in males and females by quantitative real-time PCR. The results showed that the full-length cDNA was 1389 bp in-cluding a 414 bp 5′-untranslated region (UTR), a 279 bp 3′-UTR, and a 696 bp open reading frame encoding a 231 amino acid. Asnanos1 was expressed in all tissues tested except the heart. The expression levels of the Asnanos1 gene in spleen, brain, liver, gill, and kidney were not significantly different between males and females. The ex-pression level was highest in the ovary among all tissues tested (P< 0.05). Our results provide insight into the de-velopment of all-female breeding technology for A. schrenckii.关键词
施氏鲟(Acipenser schrenckii)/nanos1基因/荧光定量RT-PCR/雌雄个体组织/基因表达Key words
Acipenser schrenckii/nanos1/real-time PCR/female and male/gene expression分类
生物科学引用本文复制引用
肖懿哲,朱友芳,孙玉龙,张鑫,张子平,王艺磊..施氏鲟Asnanos1基因序列分析及其在雌雄不同组织中表达[J].海洋科学,2017,41(4):17-23,7.基金项目
国家自然科学基金资助项目(41306174) （41306174）
福建省农业科技重点资助项目(2013N0024) （2013N0024）
莆田市科技局项目(2012N16) [The National Natural Science Foundation of China, No. 41306174 （2012N16）
Key Project in Agricultural Science and Technology of Fujian Province, No. 2013N0024 （）
Science and Technology Development Project of Putian, No. 2012N16] （）
