海洋科学2017，Vol.41Issue(4)：57-64,8.DOI:10.11759/hykx20160717001
树脂固定化海洋脂肪酶ADM47601的研究
Research on immobilization of lipase ADM47601 from marine microorganisms by resin
摘要
Abstract
The effect of different resins on immobilizing lipase ADM47601 was investigated in this study. A total of 21 types of resins were screened, and the anion-exchange resin D316 demonstrated the best immobilization. After the orthogonal test, the recovery ratio of enzyme activity reached up to 65.53% ± 1.06% under optimal conditions: 10000U lipase per 1g resin, pH7.5, 25℃, 150r/min and 3 h for immobilization. The optimal pH of immobilized lipase was 7.5, and it exhibited 85% of the maximum activity in the range of pH6.0–9.0. The optimal temperature was 40℃, and the activity was maintained at 66% after 1 h of storage at 50℃. At 25℃, the half-life was 55 days and the residual activity was still higher than 60% after utilizing the lipase 8 times. The immobilized lipase can maintain activity in various organic reagents, and its Km and Vmax values were 4.86 × 10?5mol/L and 1.31 × 10?5mol/(L·min), respectively, indicating high affinity to the substrate.关键词
脂肪酶/树脂/固定化/酶学性质Key words
Lipase/Resin/Immobilization/Enzymatic property分类
生物科学引用本文复制引用
孙国龙,马子宾,郝建华,盛军,孙谧..树脂固定化海洋脂肪酶ADM47601的研究[J].海洋科学,2017,41(4):57-64,8.基金项目
国家自然科学基金-联合基金(U1406402-5) （U1406402-5）
国际科技合作与交流专项(2014DFG30890) （2014DFG30890）
国家实验室-鳌山科技计划(2015ASKJ02-06) [National Science Foundation-Shandong province Joint Fund (U1406402-5) （2015ASKJ02-06）
International Science and Technology Cooperation and Exchanges (2014DFG30890) （2014DFG30890）
The Scientific and Technological Innovation Project Financially Supported by Qingdao National Laboratory for Marine Science and Technology (2015ASKJ02)] （2015ASKJ02）
