家畜生态学报2017，Vol.38Issue(7)：9-15,7.
中甸牦牛mtDNA ND6和COⅢ基因遗传多样性及系统进化分析
Analysis on Genetic Diversity and Phyletic Evolution of mtDNA ND6 and COⅢ Gene of Zhongdian Yak
摘要
Abstract
In order to investigate the genetic diversity and provide theoretical basis for rational utilization of Zhongdian yak,this study analyzes the complete sequence of 15 individuals' ND6 gene.We analyzed the diversity of nucleotide,composition and diversity of haploid type by MEGA5.0,DNASP5.0,Clustal X1.83,etc.The results showed that (1) the length of the ND6 gene of Zhongdian yak were 528 bp,with nucleotide frequencies of 20.8%,42.2%,7.6% and 29.4% for T,A,G,and C respectively,there are some bias of base composition.The length of the COⅢ gene of Zhongdian yak were 781 bp,with nucleotide frequencies of 29.2%,26.1%,15.2%,29.5% for T,A,G,and C respectively.(2)There are no haploid and mutant sites in 15 individuals of Zhongdian yak,showing that the genetic diversity of Zhongdian yak's ND6 gene is relatively poor;Three haplotypes were identified of 15 individuals,with four polymorphic sites,including two conversions,two transversions;(3) In evolutionary tree,Zhongdian yak and Maiwa yak clustered first,then gathered with Bos mutusc.It means there are close genetic relationship between Zhongdian yak and Bos mutusc;Known from the analysis of genetic distance,Zhongdian yak has the minimum distance with Bos mutusc,and the maximum distance with Bubalus bubalis.The result supported the point that Bos grunnuens and Bos mutus are classified as poephagus of Bovinae.关键词
中甸牦牛/ND6基因/COⅢ基因/系统进化/遗传多样性Key words
Zhongdian yak/ND6 gene/COⅢ gene/phylogeny/genetic diversity分类
农业科技引用本文复制引用
向超,钟金城,陈智华,柴志欣..中甸牦牛mtDNA ND6和COⅢ基因遗传多样性及系统进化分析[J].家畜生态学报,2017,38(7):9-15,7.基金项目
西南民族大学研究生(CX2016SZ068) （CX2016SZ068）
四川省科技厅项目(2015JY0248) （2015JY0248）
