家畜生态学报2017，Vol.38Issue(7)：20-25,6.
miR-126-5p在沙子岭猪睾丸组织差异表达分析及功能预测
Differential Expression and Function Prediction of miR-126-5p in Shaziling Boar Testis
摘要
Abstract
In this study,real-time fluorescence quantitative PCR was performed to detect miR-126-5p expression levels in eight different developmental stages (E90,D1,D30,D60,D90,D120,D150,D180) of Shaziling pig testis to explore the role of miR-126-5p in animal testicular development and spermatogenesis.The miRanda,TargetScan and mirWalk databases were used to predict the target genes of miR-126-5p,then the intersection from the three databases were taken to analyze the biological function of target genes by making use of David online software.The Real-time PCR results showed that miR-126-5p highly expressed in embryonic period (E90-D1) and with down-regulated expression in growth periods(D1-D90),while up-regulated in the sexual maturity (D120).The D1 and D90 was the highest and the lowest expression level,and they were extremely significant(P<0.01) with the other seven periods;the differences among D30,E90,D180 were not significant (P>0.05),and they had extremely significant differences (P<0.01) with the rest of the periods;the difference between D120 and D30 was significant (P<0.05),and the difference between D120 and the other periods was extremely remarkable (P<0.01);there had no difference(P>0.05) between D150 and D60,but they had bally distinct difference with the rest of periods respectively (P<0.01).The target genes of miR-126-5p involved in cell proliferation,apoptosis,differentiation,cell communication,migration and protein transport biological processes (P<0.001),the molecular functions including protein dimerization activity and ligand-dependent nuclear receptor activity (P<0.001),KEGG pathway analysis results showed that miR-126-5p involved in TGF-β and MAPK signaling pathway,focal adhesion (P<0.01).Combined the results of miR-126-5p expression differences at different developmental stages of testicular tissue with target genes function prediction,miR-126-5p may be involved in the regulation of Shaziling pig germ cell differentiation and spermatogenesis,and this study could provide a research basis for the function and regulation mechanism of miR-126-5p in spermatogenesis.关键词
沙子岭猪/miR-126-5p/生物信息学分析/精子生成Key words
Shaziling pig/miR-126-5p/bioinformatics analysis/spermatogenesis分类
农业科技引用本文复制引用
董莲花,冉茂良,翁波,李智,吴买生,陈斌..miR-126-5p在沙子岭猪睾丸组织差异表达分析及功能预测[J].家畜生态学报,2017,38(7):20-25,6.基金项目
国家现代农业产业技术体系建设专项资金(CARS-36) （CARS-36）
