吉林大学学报（理学版）2017，Vol.55Issue(4)：1025-1030,6.DOI:10.13413/j.cnki.jdxblxb.2017.04.43
重组诺如病毒P颗粒的表达纯化及免疫原性
Expression, Purification and Immunogenicity of Recombinant Norovirus P Particles
摘要
Abstract
We constructed a recombinant plasmid and expressed the purified target protein,and used SDS-PAGE,Western blot and Native-PAGE to test protein.The multimers of the target protein were analyzed by SuperdexTM 200 gel chromatography,and the particle size of the target protein was analyzed by dynamic light scattering and transmission electron microscopy.The immune response of the protein was detected by enzyme-linked immunosorbent assay with four-immunized serum.The results show that plasmid pET26b-PP-3copy-Aβ1-6-loop123 was successfully constructed,and the target protein was successfully expressed.There are three oligomers in the target protein,which are 24-mer,12-mer and 2-mer,and the particle size of the protein is about 20 nm,and has an immune response to the β-amyloid protein.关键词
诺如病毒重组P颗粒/可溶性表达/蛋白免疫原性/蛋白疫苗Key words
Norovirus recombinant P particle/soluble expression/protein immunogenicity/protein vaccine分类
生物科学引用本文复制引用
金浩,付璐,史晶莹,吴丛梅,吴慧,殷玉和..重组诺如病毒P颗粒的表达纯化及免疫原性[J].吉林大学学报（理学版）,2017,55(4):1025-1030,6.基金项目
吉林省科技发展计划项目(批准号:20150101226JC). （批准号:20150101226JC）
