吉林农业大学学报2017，Vol.39Issue(4)：449-453,5.DOI:10.13327/j.jjlau.2017.3760
黄芪多糖和枯草芽孢杆菌代谢产物在PK-15细胞上对TGEV的影响
Effects of Metabolites of Astragalus polysaccharide and Bacillus subtilis on TGEV of PK-15
摘要
Abstract
In order to study the effects of metabolic products of astragalus polysaccharide and Bacillus subtilis on anti-infectious gastroenteritis virus (TGEV) in PK-15 cells,metabolites of Bacillus subtilis,Astragalus polysaccharides and complexes were added to TGEV infected PK-15 cells.MTT assay was used to detect viral inhibition rate.The effects of the metabolic products of Bacillus subtilis and Astragalus polysaccharide on TGEV N and IFN-γ mRNA expression were detected by fluorescence quantitative PCR.The results showed that metabolites of Bacillus subtilis had anti-TGEV effect,Astragalus polysaccharide could inhibit TGEV infection of PK-15 cells individually,and viral inhibition rate increased significantly after the union.The results of fluorescence quantitative PCR showed that expression of TGEV N gene in PK-15 cells treated by the joint was significantly de creased,expression of IFN-γ mRNA in PK-15 cells was significantly increased in 3 h and 6 h.Metabolic products of Bacillus subtilis have significant anti-TGEV effect on PK-15 cell platforms,and Astragalus polysaccharide also has a remarkable anti-TGEV effect.关键词
枯草芽孢杆菌代谢产物/黄芪多糖/猪传染性胃肠炎病毒Key words
Bacillus subtili metabolite/Astragalus polysaccharide/TGEV分类
农业科技引用本文复制引用
汪帅男,王璐,张萍,魏萍..黄芪多糖和枯草芽孢杆菌代谢产物在PK-15细胞上对TGEV的影响[J].吉林农业大学学报,2017,39(4):449-453,5.基金项目
黑龙江省教育厅重点项目(12521z001),哈尔滨市科技局项目(2014RFXGJ096),黑龙江省科技厅项目(201007),农业部动物疫病病原生物学学科群东北科学观测实验站项目(201108) （12521z001）
