吉林农业大学学报2017，Vol.39Issue(4)：477-482,487,7.DOI:10.13327/j.jjlau.2016.3191
响应面优化超声波辅助酶法提取小米蛋白工艺
Optimization of Millet Protein Ultrasonic-assisted Enzymatic Extraction Technology by Response Surface Methodology
摘要
Abstract
Optimum process conditions of enzymatic extraction of protein from millet were determined.Ultrasonic-assisted method was used to extract protein from millet,and the effects of enzyme addition amount,enzymatic hydrolysis temperature,ultrasonic power,ultrasonic time and enzymatic hydrolysis time on extraction yield of protein were studied by single factor experiments.On the basis of single factor experiments,enzymatic hydrolysis temperature,ultrasonic power and enzyme addi tion amount were selected as the main factors influencing extraction yield.Optimum conditions were optimized by response surface method using extraction yield as the evaluation index,and mathematical regression models were established.Optimum conditions of ultrasonic-assisted extraction of protein from millet were:2.5％ enzymatic addition amount,enzymatic hydrolysis temperature 43 ℃,ultrasonic power 420 W,ultrasonic time 25 min,enzymatic hydrolysis time 100 min,under which extraction yield was 43.26％,significantly higher than that of univariate extraction.关键词
小米/蛋白质/超声波/酶/响应面法Key words
millet/protein/ultrasonic/enzyme/response surface methodology分类
轻工纺织引用本文复制引用
杨桦,刘振春,刘想,崔晶蕾..响应面优化超声波辅助酶法提取小米蛋白工艺[J].吉林农业大学学报,2017,39(4):477-482,487,7.基金项目
公益性行业(粮食)科研专项(201313011-3) （粮食）
