江苏大学学报（医学版）2017，Vol.27Issue(4)：287-290,4.DOI:10.13312/j.issn.1671-7783.y170024
伤寒沙门菌长链非编码RNA AS-RpoH分子鉴定
Identification of non-coding RNA AS-RpoH in Salmonella enterica serovar Typhi
摘要
Abstract
Objective: To clarify the molecular characteristics of a non-coding RNA, AS-RpoH in Salmonella enterica serovar Typhi(S.Typhi).Methods: Northern blotting analysis was used to investigate the expression of AS-RpoH in S.Typhi;RT-PCR was used to identify the possible transcription initiation and termination sites of AS-RpoH;Northern blotting analysis with specific probe was used to analyze the transcripts of yhhK.Results: The AS-RpoH expression was confirmed by Northern blotting with a length of approximate 3 000 nt;The transcription termination site of AS-RpoH was located in the region between 191-238 nucleotides of rpoH start codon upstream;The possible initiation site of AS-RpoH transcription was located on the upstream of yhhK;Northern blotting analysis revealed that the length of yhhK transcript was the same as the length of AS-RpoH.Conclusion: AS-RpoH is one of long non-coding RNAs expressed in S.Typhi, which may be a possible 3′-untranslation region of the yhhK.关键词
伤寒沙门菌/非编码RNA/AS-RpoH/分子鉴定/yhhKKey words
Salmonella enterica serovar Typhi/non-coding RNA/AS-RpoH/molecular identification/yhhK分类
医药卫生引用本文复制引用
李雪娇,刘锐,熊昌艳,赵昕,黄新祥..伤寒沙门菌长链非编码RNA AS-RpoH分子鉴定[J].江苏大学学报（医学版）,2017,27(4):287-290,4.基金项目
国家自然科学基金资助项目(81371780) （81371780）
江苏省研究生创新计划资助项目(CXLX13_692) （CXLX13_692）
