实用临床医药杂志2017，Vol.21Issue(13)：17-21,5.DOI:10.7619/jcmp.201713005
表没食子儿茶素没食子酸酯对同型半胱氨酸促血管平滑肌细胞增殖的保护作用
Protective effects of epigallocatechin gallate on homocysteine-induced proliferation of vascular smooth muscle cells
摘要
Abstract
Objective To observe the protective effect of epigallocatechin gallate(EGCG)on homocysteine-induced proliferation of human aortic smooth muscle cells(HASMCs).Methods HASMCs were served as objects,which were cultured in various media,including Hcy(100,200,500,1 000 μmol/L),EGCG(EGCG 5,10,20 μmol/L),Hcy+EGCG(Hcy 500 μmol/L+EGCG 5 μmol/L,Hcy 500 μmol/L+EGCG 10 μmol/L,Hcy 500 μmol/L+EGCG 20 μmol/L)for 24 h.The proliferation of HASMCs was determined by cell counting kit-8(CCK-8)and bromodeoxyuridine(BrdU)labeling.The level of angiotensin Ⅱ(AngII)was determined by enzyme-linked immunosorbent assay(ELISA),and the level of angiotensin Ⅱ type 1 receptor(AT-1R)was determined by Western blot.Results After treatment for 24 h,compared with the control group,the proliferation of cells in different Hcy groups significantly increased(P<0.05),while the proliferation of cells in EGCG 10 and 20 μmol/L group significantly reduced(P<0.05).Compared with the Hcy 500 μmol/L group,cell proliferation significantly reduced after adding EGCG 10 and 20 μmol/L(P<0.01),while the expression levels of AngII and AT-1R decreased significantly(P<0.01).Conclusion EGCG can inhibit Hcy-induced proliferation of VSMCs.关键词
同型半胱氨酸/表没食子儿茶素没食子酸酯/增殖/血管紧张素Ⅱ/血管紧张素Ⅱ1型受体Key words
homocysteine/epigallocatechin gallate/proliferation/angiotensinII/angiotensin Ⅱ type 1 receptor分类
医药卫生引用本文复制引用
战晓丽,周东池,顾惠芳,杨秀红,金惠敏..表没食子儿茶素没食子酸酯对同型半胱氨酸促血管平滑肌细胞增殖的保护作用[J].实用临床医药杂志,2017,21(13):17-21,5.基金项目
上海市浦东新区卫生系统优秀青年医学人才培养资助项目(PWRq2014-05) （PWRq2014-05）
上海市医学重点专科建设项目(ZK2015A15) （ZK2015A15）
上海市浦东新区卫生系统重点学科建设项目(PWZx2014-11) （PWZx2014-11）
上海市浦东新区医学领先人才项目(PWRI2012-05) （PWRI2012-05）
