江西农业学报2017，Vol.29Issue(6)：66-69,74,5.DOI:10.19386/j.cnki.jxnyxb.2017.06.15
槟榔内生真菌的分离与初步鉴定
Isolation and Preliminary Identification of Endophytic Fungi from Arecanut (Areca catechu)
摘要
Abstract
In order to understand the diversity of endophytic fungi in arecanut (Areca catechu L.),we used tissue block culture method to isolate and identify 47 endophytic fungal strains from the mot,leaf and flower of arecanut,amplified the rDNA-ITS sequence of these strains,and constructed their phylogenetic tree.The results showed that the obtained 47 endophytic fungal strains belonged to 8 genera,including Penicillium,Fusarium,Phyllosticta,Curvularia,Nigrospora,Colletotrichum,Acremonium and Exserohilum.Among these genera,Penicillium and Fusarium were the dominant genera,and the endophytic fungal strains in these 2 genera accounted for 31.91％ and 27.66％ of total isolated strains,respectively.关键词
槟榔/内生真菌/分离/鉴定/rDNA-ITS序列/系统发育分析Key words
Arecanut/Endophytic fungi/Isolation/Identification/rDNA-ITS sequence/Phylogenetic analysis分类
农业科技引用本文复制引用
宋薇薇,牛晓庆,余凤玉,朱辉,唐庆华,覃伟权..槟榔内生真菌的分离与初步鉴定[J].江西农业学报,2017,29(6):66-69,74,5.基金项目
海南省自然科学基金项目“内生菌对槟榔黄化病发生危害的影响”(314144) （314144）
海南省重点研发计划项目“槟榔拮抗内生菌资源的筛选与利用”(ZDYF2016058). （ZDYF2016058）
