江西农业学报2017，Vol.29Issue(7)：40-43,4.DOI:10.19386/j.cnki.jxnyxb.2017.07.09
蔗糖浓度对高温下大棚早生新水梨花粉活力的影响
Effect of Sucrose Concentration on Pollen Viability of "Zaoshengxinshui"Pear in Greenhouse at High Temperature
摘要
Abstract
The author measured the pollen viability of"Zaoshengxinshui"pear at flower bud stage in greenhouse at different high temperatures (25,35 and 40 ℃)by adopting the isolated germination method,and studied the effects of different sucrose concentrations (10%,15% and 20%)in culture medium on the tolerance of pollens to high temperature,in order to screen out the optimum sucrose concentration for pollen viability assay,and to provide a guidance for artificial supplementary pollination and theoretical basis for high temperature control in facility cultivation. The results showed that the solid culture medium containing 15% sucrose was more suitable for pollen germination in vitro,but the optimum sucrose concentration for pollen tube growth was 10%."Zaoshengxinshui"pear at flower bud stage in greenhouse could resist to the high temperature of 35 ℃,but its pollen ger-mination rate began to decrease when the temperature kept at 35 ℃ for 2 h. At 40 ℃ high temperature,the pollen germination rate was significantly reduced. In addition,the damage of high temperature to the pollen viability could not be repaired,while high temperature had little influence on the length of pollen tube. So,15% sucrose in solid culture medium was the best for pollen ger-mination assay in vitro. High temperature could obviously reduce the pollen vitality,and the long-time high temperature above 35℃ should be avoided in the facility cultivation of pear.关键词
蔗糖/大棚/梨/高温/花粉萌发/花粉管Key words
Sucrose/Greenhouse/Pear/High temperature/Pollen germination/Pollen tube分类
农业科技引用本文复制引用
王晓庆,骆军,施春晖,张学英,蒋爽,滕美贞,张玲..蔗糖浓度对高温下大棚早生新水梨花粉活力的影响[J].江西农业学报,2017,29(7):40-43,4.基金项目
上海市果业产业技术体系[沪农科产字(2016)第7号] （2016）
国家科技部星火计划项目(2012GA680002). （2012GA680002）
