检验医学与临床2017，Vol.14Issue(13)：1864-1866,3.DOI:10.3969/j.issn.1672-9455.2017.13.007
不同稀释液在乙型肝炎病毒DNA稀释检测中的应用研究
The application of evaluation of the different diluents used in the HBV-DNA diluted detection
摘要
Abstract
Objective Analysis and evaluation the results of HBV-DNA diluted detection using different diluents,to evaluation the application value of those diluents in diluted detection of high loads sample.Methods 30 samples which the first detection of HBV-DNA loads exceed the linear scope were collected,compared the difference between results when use negative quality product,negative serum,physiological saline,distilled water as diluents.Results In time of diluted detection of those high loads sample,there was no significant difference between the results(F=1.198,P=0.314) when use negative quality product,negative serum,physiological saline,distilled water as diluents;the TEa were within the allowed range,but the difference between bias was statistically significant(F=16.945,P<0.05);the bias was minimum in time of use distilled water as diluents,and the results of distilled water diluted detection had good linear relationship between that of negative quality product diluted detection,the fitting equation was Y=0.970X+0.255(Y=results of negative quality product diluted detection,X=results of distilled water diluted detection),R2=0.959.Conclusion In time of negative quality product could not meet the need of diluted detection,using distilled water as diluents could achieve satisfactory results.关键词
稀释液/乙型肝炎病毒/脱氧核糖核酸Key words
diluents/Hepatitis B/DNA引用本文复制引用
汪东剑,彭慧琼,彭国林,林萍,徐波,刘仙..不同稀释液在乙型肝炎病毒DNA稀释检测中的应用研究[J].检验医学与临床,2017,14(13):1864-1866,3.基金项目
南京军区医学科技创新课题项目(MS078). （MS078）
