摘要
Abstract
Objective To evaluate the clinical diagnosis value of T-lymphocyte cell activenss in bronchoalveolar lavage fluid(BALF)of patients with several common pulmonary diseases.Methods 61 cases of inpatients in this hospital from 2013 to 2015 were selected,including 15 cases of lung cancer,10 cases of pulmonary tuberculosis,13 cases of sarcoidosis,15 cases of pulmonary infection,8 cases of idiopathic pulmonary interstitial fibrosis(IPF).The levels of T lymphocyte subsets in BALF were detected by flow cytometry.Results CD3+,CD4+,CD8+ and CD4+/CD8+ were obviously changed in the group of lung cancer and sarcoidosis,the difference was statistically significant(P<0.05).In the group of lung cancer,CD3+ and CD4+ were lowered,CD8+ was increased,and CD4+/CD8+ was significantly lowered.However,in the group of sarcoidosis,CD4+ was increased,CD8+ was lowered,and CD4+/CD8+ was significantly increased.In addition,in the group of tuberculosis and pulmonary infection,CD4+ was lowered,CD8+ was comparatively increased,and CD4+/CD8+ was significantly lowered,they were significantly different from sarcoidosis and IPF(P<0.05).In the group of IPF,CD3+,CD4+,CD8+ and CD4+/CD8+ were closed to normal value.Conclusion Detecting the level of T-lymphocyte subsets in BALF helps differentiating the above-mentioned pulmonary diseases effectively to some extent,thus it has important value in exploring the immune function of patients and disaese differential diagnosis and prognosis.关键词
流式细胞技术/肺泡灌洗液/T淋巴细胞亚群Key words
flow cytometry/bronchoalveolar lavage fluid/T-lymphocyte subsets
