检验医学与临床2017，Vol.14Issue(Z1)：4-7,4.DOI:10.3969/j.issn.1672-9455.2017.25.002
丙肝患者干扰素治疗后并发AITD的自身抗体变化特点研究
The changes of autoantibodies in patients with hepatitis C virus related AITD after interferon treatment
摘要
Abstract
Objective To investigate the changes of autoantibodies in patients with HCV related AITD after interferon treatment.Methods 300 cases of HCV patients from our hospital afer the interferon therapy from January 1,2016 to October 31.contained in this research Using enzymatic method to detect the levels of patients′Aspertate aminotransferase(Aspertate aminotransferase,AST),alanine aminotransferase(Alamine aminotransferase,ALT),total bilirubin(TBil) and gamma-glutamyl transpeptidase(GGT).Real-time fluorescent quantitative PCR method was used to detect HCV RNA.Luminescence method was used to detect anti-thyroid autoantibodies.Application of indirect immunofluorescence technique to detect autoantibodies and western blot method to detect the ANA antibody spectrum.Results The induced AITD after interferon treatment of HCV patients group(AITD) was mostly females,the levels of ALT,AST,TBILand GGT were higher than that with non-AITD HCV patients group,the difference was statistically significant(P<0.05).The positive rate(97.5%) that at least one antibody positive of AITD patients with five kinds of autoantibodies(ANA,PCA,SMA,AMA,LKM) was higher than non-AITD group patients′.The ANA has the highest positive rate(60.5%) among the above five kinds of autoantibodies,in AITD patients,which significantly higher than that in the non-AITD group,the difference was statistically significant(P<0.05).The highest percentage of spectrum in ANA antibody of AITD patients was the homogeneous type which accounting for 22.0%.In ANA affirm experiment,RO-52 occupied the highest proportion(10.8%),and followed by CENP-B(3.2%).Conclusion There were higher rates of variety of autoantibodies in serum in HCV related AITD patients than in non-AITD patients,which indicated that HCV related AITD is associated with multiple autoimmune mechanisms and immune damage.关键词
丙型病毒性肝炎/干扰素/自身免疫性甲状腺病/自身抗体Key words
hepatitis C virus/interferon/autoimmune thyroid disease/autoantibodies引用本文复制引用
李妍,黄加干,杨宁,谢娜,张岚,洪炜,朱剑功,李伯安,王晗..丙肝患者干扰素治疗后并发AITD的自身抗体变化特点研究[J].检验医学与临床,2017,14(Z1):4-7,4.基金项目
北京市自然科学基金青年项目(7154239). （7154239）
