河南理工大学学报（自然科学版）2017，Vol.36Issue(3)：79-85,7.DOI:10.16186/j.cnki.1673-9787.2017.03.013
一株好氧反硝化菌的筛选鉴定与特性研究
Screening and characterization study on an aerobic denitrifying bacteria
摘要
Abstract
For screening efficient aerobic denitrifying bacteria,it is studied the suitable growth conditions and the performance of aerobic denitrification,and it is finally provided the theory basis for the biological denitrification about application of the bacteria in the water,using flat line,morphology observation,physiological and biochemical and 16S rDNA sequence analysis screening to denitrify the genus status of bacteria and examine denitrification performance of the strain under the growth condition of the different nitrogen source,carbon source,pH,temperature,cultivation strains.Efficient denitrifying bacteria QX1 section identified as Arthrobacter sp..The bacteria under the gas bath shaking table speed 160 r/min is that LB medium + quality volume ratio of 1％ acetic acid sodium,the optimal temperature 30 ℃,the optimum pH value 8,strains of alkali,temperature 15 ℃ at low temperature tolerance and 40 ℃ at high temperature tolerance.The growth curve showed that under the condition of suitable cultivation,strains grew into the index after 4 h period,this period maintained about 16 h,strains entered the decline phase after 24 h stable growth,and strains in the synthetic wastewater aerobic training after 48 h,nitrate nitrogen removal rate could reach 89.92％.The bacteria QXI could be used as alkali resistance,well low temperature resistance and high temperature resistance of efficient denitrification alternative strains,developing low or high temperature area water quality improver would have broad application prospects.关键词
好氧反硝化/节杆菌/筛选鉴定Key words
aerobic denitrification/Arthrobacterium/isolation and identification分类
资源环境引用本文复制引用
孙玲,葛清莲,曹文平,张惠芳,张建昆,张慧,董玉玮,李倩囡,汪银梅..一株好氧反硝化菌的筛选鉴定与特性研究[J].河南理工大学学报（自然科学版）,2017,36(3):79-85,7.基金项目
住房与建设部科技计划项目(2015-K6-014,R22016061) （2015-K6-014,R22016061）
江苏省六大人才高峰科研项目(JNHB-005) （JNHB-005）
江苏省高校自然科学基金资助项目(13KJD610002) （13KJD610002）
徐州工程学院校级科研项目(XKY2015309) （XKY2015309）
