临床口腔医学杂志2017，Vol.33Issue(7)：397-400,4.DOI:10.3969/j.issn.1003-1634.2017.07.005
口腔扁平苔藓关键转录信号蛋白的表达差异分析
Characteristic expression of the critical transcription factors in the OLP
摘要
Abstract
Objective:To screen the key transcription signaling proteins,associated with the epithelium apoptosis and inflammation in the OLP,and to understand the pathogenesis of OLP.Methods:OLP patients(n=8)and the normal control group(n=6)were collected,then oral mucosa lesion,normal buccal mucosa and peripheral blood from each group were captured.The concentration of the protein from each specimen was determined after it was extracted.Samples were added in the plate according to the kit instruction,then the results were scaned by using the Odyssey? infrared imaging system.Finally,the average RFU value of every sample was analyzed by using the LI-COR? Image Studio v2.0 chip analysis software.Results:p38 MAPK(Thr180/Tyr182)expression increased significantly in the reticular OLP(both P＜0.001)compared to the control and erosive mucosa.However,in the erosive lesions,compared with the control and reticular mucosa,STAT3(Ser727)expression was significantly elevated(P＜0.001 and P=0.002 respectively).Meanwhile,STAT3(Ser727)expression in the reticular OLP was higher compared to the normal control(P=0.001).In addition,the expression of p38 MAPK and STAT3 increased significantly in the PBMCs from the OLP patients,compared with those from the normal controls(P=0.001 and P=0.006 respectively).Conclusion:p38 MAPK(Thr180/Tyr182)and STAT3(Ser727),as important signal transduction factors,might be involved in the process of OLP,especially account for the different clinical manifestations of OLP.关键词
口腔扁平苔藓/STAT3/p38MAPK/临床分型Key words
Oral lichen planus/STAT3/p38MAPK/Clinical type分类
医药卫生引用本文复制引用
杜观环,王宇峰,曹天翼,周磊磊,唐国瑶..口腔扁平苔藓关键转录信号蛋白的表达差异分析[J].临床口腔医学杂志,2017,33(7):397-400,4.基金项目
国家自然科学基金(81570975,81400512) （81570975,81400512）
国家临床重点专科建设项目([2013]544) （[2013]544）
上海市口腔医学研究所院级基金(院2016-13) （院2016-13）
