临床口腔医学杂志2017，Vol.33Issue(7)：403-406,4.DOI:10.3969/j.issn.1003-1634.2017.07.007
人脐带间充质干细胞条件培养基对舌鳞癌Cal-27细胞增殖的影响
Effects of human umbilical cord mesenchymal stem cell conditioned medium on proliferation of tongue squamous cell carcinoma Cal-27
摘要
Abstract
Objective:To investigate the effect of human umbilical cord mesenchymal stem cells(HUC-MSCs)on the biological characteristics of tongue squamous cell carcinoma Cal-27 cells,and to provide a new idea for the treatment of TSCC.Methods:HUC-MSCs and Cal-27 cells were cultured and observed under inverted microscope.The proliferative activity of Cal-27 cells was detected by CCK-8 method.The proliferation of Cal-27 cells was observed by co-culture of HUC-MSCs conditioned medium with Cal-27 cells.The expression of apoptotic protein and Wnt / β-catenin signaling pathway in Cal-27 cells was detected by Western blot.Results:The results of growth curve showed that HUC-MSCs could inhibit the proliferation of Cal-27 cells in a dose-dependent manner.These changes were associated with up-regulation of PARP-1 expression in Cal-27 cells,and Bcl-2 and β-catenin,C-Myc expression down.Conclusion:HUC-MSCs conditioned medium can inhibit the proliferation and induce apoptosis of Cal-27 cells,which may be related to the inhibition of Wnt / β-catenin signaling pathway.关键词
人脐带间充质干细胞/条件培养基/舌鳞癌细胞/增殖/凋亡/Wnt信号通路Key words
Human umbilical cord mesenchymal stem cells(HUC-MSCs)/Conditioned medium/Tongue squamous cell carcinoma/Proliferation/Apoptosis/Wnt signaling pathway分类
医药卫生引用本文复制引用
徐玥,李涛,董刚,李凤梅,于珊珊,李娜娜,黄磊,郑建金..人脐带间充质干细胞条件培养基对舌鳞癌Cal-27细胞增殖的影响[J].临床口腔医学杂志,2017,33(7):403-406,4.基金项目
青岛市科技局重点项目(15-9-2-79-nsh) （15-9-2-79-nsh）
