环境与职业医学2017，Vol.34Issue(6)：536-541,6.DOI:10.13213/j.cnki.jeom.2017.16678
纳米二氧化硅对A549细胞的毒性作用
Cytotoxicity of silica nanoparticles in A549 cells
摘要
Abstract
[Objective] To study the toxic effects of SiO2 nanoparticles (nano-SiO2) on human pulmonary adenocarcinoma A549 cells.[Methods] Different concentrations of nano-SiO2 (10-20nm) (5,10,25,50,and 100mg/L) were evaluated for their potential toxicity in A549 cells.Cell viability was determined by MTT assay,lactate dehydrogenase (LDH) activity by colorimetric analysis method,microstructure of organelle by transmission electron microscope,and apoptosis and cell cycle by flow cytometry.[Results] Compared with the control group,exposure to increasing concentrations of nano-SiO2 resulted in a diminishing viability of A549 cells.The activity of LDH increased in a dose-dependent manner with nano-SiO2 from 0 to 25 mg/L (P＜0.01 or P＜ 0.05),decreased at 50mg/L nano-SiO2,and then increased at 100 mg/L nano-SiO2.With the increase of nano-SiO2 concentrations,cell membrane villi decreased;organell cavity changes increased;secondary lysosomes increased;more cell apoptosis or necrosis appeared;A549 cells were arrested at G0/G1 phase and proliferation was inhibited.[Conclusion] Nano-SiO2 can be uptaken by A549 cells,resulting in impaired organelle structure and function,thus inhibiting cell proliferation,inducing cell apoptosis,and causing cell cyclical disorders.关键词
二氧化硅/细胞毒性/A549细胞/纳米材料/增殖/凋亡/乳酸脱氢酶Key words
silicon dioxide/cytotoxicity/A549 cell/nanoparticle/proliferation/apoptosis/lactate dehydrogenase分类
医药卫生引用本文复制引用
孙悦,刘嘉祺,王路,彭子荷,刘和亮,郝小惠..纳米二氧化硅对A549细胞的毒性作用[J].环境与职业医学,2017,34(6):536-541,6.基金项目
国家自然科学基金项目(编号:81673119) （编号:81673119）
华北理工大学大学生创新项目(编号:X2016213) （编号:X2016213）
唐山市老年医学科技创新团队(编号:15130212C) （编号:15130212C）
