环境与职业医学2017，Vol.34Issue(6)：542-544,3.DOI:10.13213/j.cnki.jeom.2017.16812
亚砷酸钠对雄性大鼠炎症因子和氧化应激的影响
Effects of sodium arsenite on inflammatory cytokines and oxidative stress in male rats
摘要
Abstract
[Objective] To investigate the effects of sodium arsenite on serum inflammatory cytokine and oxidative stress levels in male rats.[Methods] Thirty-two specific-pathogen-free healthy male SD rats were randomly divided into four groups:control group,low dose,medium dose,and high dose groups,with eight rats in each group.The three exposure groups were intoxicated by 10,100,and 1000 μg/L NaAsO2 in drinking water,respectively,while the control group was given ultrapure water for free drinking.All the rats were sacrificed after 28 days.The levels of granulocyte-macrophage colony stimulating factor (GM-CSF),interleukin-10 (IL-10),nicotinanmide adenine dinucleotide phosphate (NADPH) oxidase,reactive oxygen species (ROS),total antioxidant capacity,and nitric oxide (NO) were determined by enzyme-linked immunosorbent assay.[Results] Sodium arsenite exposed rats showed significantly higher GM-CSF expression levels than the control group (P＜0.05),and the 1000 μg/L group had the highest GM-CSF expression level.The anti-inflammatory cytokine IL-10 levels were lower in all exposure groups than in the control group (P ＜0.05).The 1000 μg/L group showed a higher ROS level than the control group (P＜ 0.05).NO expression levels were increased in all exposure groups compared with the control group (P＜0.05),and there was a dose-response relationship.[Conclusion] Sodium arsenite exposure in male rats could lead to inflammatory injury and oxidative stress injury,and the mechanism may be related to elevated NO and reduced IL-10.关键词
砷/亚砷酸钠/炎症因子/氧化应激Key words
arsenic/sodium arsenite/inflammatory cytokine/oxidative stress分类
医药卫生引用本文复制引用
杨欢,王素华,苏鑫,高艳荣..亚砷酸钠对雄性大鼠炎症因子和氧化应激的影响[J].环境与职业医学,2017,34(6):542-544,3.基金项目
国家自然科学基金项目(编号:81660532) （编号:81660532）
内蒙古科技计划项目(编号:201502080) （编号:201502080）
