老年医学与保健2017，Vol.23Issue(3)：189-192,4.
红景天苷对Aβ1-40诱导PC12细胞损伤的保护作用及机制
Protective Effect of Salidroside on PC12 Cell Injury Induced by Aβ1-40 and the Mechanism
摘要
Abstract
Objective To observe the effect of salidroside on PC12 cell injury induced by Aβ1-40 and to explore its mechanism of improving energy metabolism disorder in the development of Alzheimer's disease.Methods PC12 cells were treated with Aβ1-40to build an AD cell model;CCK-8 method was applied in the measurement of cell viability and an ATP assay kit in the measurement of adenosine triphosphate (ATP) level;the expression level of PGC-1α was detected via immunofluorescence assay and Western blot analysis.Results Salidroside inhabited the decrease of PC 12 cell viability induced by Aβ1.40 (P＜0.05) and increased the level of ATP (P＜0.01);the results of innunofluorescence assay and Western blot analysis indicated that salidroside up-regulated the expression of PGC-1α (P＜0.05).Conclusions Salidroside may inhibit Aβ1-40-induced PC12 cells injury by up-regulating the expression of PGC-1α,an energy metabolism related protein,and increasing the level of ATP,it may be of certain curative effect on Alzheimer's disease.关键词
β-淀粉样蛋白1-40/阿尔茨海默病/红景天苷/三磷酸腺苷Key words
Aβ1-40/Alzheimer's disease/salidroside/adenosine triphosphate (ATP)引用本文复制引用
黄旭娇,邢三丽,陈川,郁志华,陈久林..红景天苷对Aβ1-40诱导PC12细胞损伤的保护作用及机制[J].老年医学与保健,2017,23(3):189-192,4.基金项目
国家自然科学基金(81503626) （81503626）
上海市卫生和计划生育委员会青年基金(201540254) （201540254）
