林业科学2017，Vol.53Issue(3)：21-32,12.DOI:10.11707/j.1001-7488.20170303
青海不同林分土壤微生物群落结构(PLFA)
Profile of Soil Microbial Community under Different Stand Types in Qinghai Province
摘要
Abstract
[Objective] Seven natural stand types were investigated to understand the soil microbial community.The main forest species were Picea crassifolia,Betula platyphylla,Larix gmelinii,Populus davidiana.The purpose of this study was to improve management and evaluation strategies of the forest by adjusting the structure and restoring the degraded forest.[Method] The 7 stand types were Datong Picea crassifolia (A),Datong Betula platyphylla (B),Huangzhong Picea crassifolia + Betula platyphylla (C),Ledu Larix gmelinii + Betula platyphylla (D),Minhe Populus davidiana (E),Xunhua Populus davidiana + Betula platyphylla (F),and Jianzha Picea crassifolia (G) in Qinghai Province.The soil physical-chemical properties and soil microbial community composition were investigated by conventional laboratory analysis and phospholipid fatty acids (PLFAs) analysis.Changes of individual PLFA signatures and correlations between soil properties and soil microbial group of PLFA indicators were analyzed by principal components analysis (PCA) and redundancy analysis (RDA),respectively.[Result] A total of 17 different PLFAs with different types of biomarkers were detected in the soil samples among different stand types.The stand types A and B exhibited a larger number PLFAs compared with other stand types.The lowest number of PLFAs was found in stand type G.The PLFAs biomarker was variable in different stand soils.The highest content was 16:0.The highest richness of PLFAs was saturated fatty acid.The highest total content of PLFAs biomarkers was found in stand B,and the lowest in stand G.The contents of bacteria and fungus PLFAs displayed the following order:broad-leaved stand ＞ mixed broadleaf-conifer stand ＞conifer stand.The Simpson index,Shannon-Wiener index of stands F and G were significantly lower than those of the other stand types.McIntosh index were holistically higher in stand types A,B and D than in the other types.Principal Component Analysis (PCA) showed that generalized bacteria and Gram positive bacteria were the main soil microbial group.Redundancy analysis (RDA) indicated that the effects of pH,soil moisture and fine root biomass on soil microbial community were higher than those of soil organic carbon,bulk density and litter standing crop.[Conclusion] Soil microbial community composition and impact factors were significantly different among different stand types.Therefore,the management and utilization of forest ecosystem should consider the change of soil microbial community characteristics,in order to improve forest management practices.关键词
磷脂脂肪酸(PLFAs)/微生物群落/林型/土壤有机碳/青海Key words
phospholipid fatty acids (PLFAs)/microbial community/stand type/soil organic carbon/Qinghai Province分类
农业科技引用本文复制引用
字洪标,向泽宇,王根绪,阿的鲁骥,王长庭..青海不同林分土壤微生物群落结构(PLFA)[J].林业科学,2017,53(3):21-32,12.基金项目
中国科学院战略性先导科技专项“应对气候变化的碳收支认证及相关问题”(XDA05050207) （XDA05050207）
国家自然科学基金项目(31370542) （31370542）
中央高校基本科研业务费优秀科研团队及重大孵化项目(2014NZYTD01). （2014NZYTD01）
