林业科学2017，Vol.53Issue(6)：37-46,10.DOI:10.11707/j.1001-7488.20170605
基于SSR标记的木荷核心种质构建
Construction of Core Collection of Schima superba Based on SSR Molecular Markers
摘要
Abstract
[Objective] The most suitable sampling strategy and proportion for core collection of Schima superba were defined through a comparative analysis and evaluation,and a core collection of S.superba was constructed.Molecular identity of the core collection was further studied,providing theoretical basis and core materials for further research and utilization of S.superba germplasms.It also provides a basis for the core collection construction of other woody plants.[Method] Taking 754 accessions of S.superba as materials that were come from seven provinces of China.The methods of M strategy (maximization strategy,M),random sampling (R),simulated annealing algorithm maximizing the genetic diversity (SAGD) and simulated annealing algorithm maximizing the number of alleles (SANA) were used to construct the core collections using 13 SSR primer pairs.The parameters of genetic diversity,such as number of alleles (Na),effective number of alleles (Ne) and Shannon''s information index (I),were used to determine the optimal method.[Result] 128 alleles (Na) were detected in 13 SSR primer pairs,the average of Ne was 3.47.High genetic diversity was revealed in the germplasm resources of S.superba (I=1.39).Comparative analysis showed that the core collection constructed by SANA,SAGD and M strategies were all better than R strategy.The retained of Na in the core collection was low,although the core collection with SANA and SAGD are better representation of the original collection.The retained of Na in the core collection that was constructed by M strategy was apparently higher than other core collections that were constructed by other three strategies.According to the parameters of genetic diversity,and the number of sampling,the core collection constructed by M strategy could preserve the greatest level of genetic diversity of germplasm resources with the minimum sample size,indicating that M strategy was optimal in terms of establishing the most representative core collection,although SANA and SAGD methods all have good representation.The principal coordinate analysis (PCoA) showed that the core collection constructed by M strategy could more comprehensively represent at the genetic diversity level of S.superba.The 115 accessions of S.superba includes 15.3% samples of the germplasms,the retention ratio of Na,Ne and I were 93.8%,115.6% and 109.9%,and the specific molecular identity for 115 core collections were established with 99.99% probability of confidence.These results demonstrated that the 115 accessions could stand for the initial collection,at the same time a set of unique molecular identity was established for 115 accessions based on 13 SSR loci.[Conclusion] M strategy is a suitable method for constructing S.superba core collection.These results demonstrated that the 115 accessions as core collection could represent the original germplasms,at the same time this research method of the construction of core collection would provide an example for other tree species.关键词
木荷/SSR标记/核心种质/分子身份信息Key words
Schima superba/SSR marker/core collection/molecular identity分类
农业科技引用本文复制引用
杨汉波,张蕊,王帮顺,徐肇友,周志春..基于SSR标记的木荷核心种质构建[J].林业科学,2017,53(6):37-46,10.基金项目
"十二五"国家科技支撑计划课题(2012BAD01B04) （2012BAD01B04）
浙江省竹木农业新品种选育重大科技专项竹木育种协作组项目(2012C12908-6) （2012C12908-6）
福建省林木种苗科技攻关五期项目木荷课题 （）
江西省林业厅林业科技创新专项项目(201503). （201503）
