林业科学2017，Vol.53Issue(6)：85-93,9.DOI:10.11707/j.1001-7488.20170610
泡桐丛枝病发生与代谢组变化的关系
Relationship between Metabolites Variation and Paulownia Witches' Broom
摘要
Abstract
[Objective] Paulownia witches'' broom caused by phytoplasma can lead to severe damage to the survival and wood production of Paulownia trees,which can result in a significant economic loss.This study aims to explore the relationship between metabolites variation and Paulownia witches'' broom using metabolomic analysis.[Method] High performance liquid chromatography-mass spectrometry (HPLC-MS) were used to analyze variation in the metabolite of healthy and phytoplasma-infected Paulownia tomentosa seedlings,differentially expressed m/z peaks were selected using principal component analysis and partial least squares-discriiminate analysis,then the differentially expressed m/z peaks detected by MS were mapped to KEGG metabolites database to find the changes of metabolite content and species in response to phytoplasma infection.[Result] The result showed that 1 612 metabolites were differentially expressed,among which 765 metabolites have a decreased concentration in phytoplasma-infected seedlings compared to healthy seedlings,and 847 metabolites are on the contrary.KEGG pathway analysis showed that 460 differentially expressed metabolites were mapped to 111 KEGG pathways.The most significantly represent category among the assigned pathways was "isoquinoline alkaloid biosynthesis",followed by "diterpenoid biosynthesis" and "flavonoid biosynthesis".Metabolites which are involved in plant hormones signal transduction and flavonoid biosynthesis were identified to be related to plant''s response to phytoplasma infection.Among them,the concentrations of zeatin,zeatin riboside,gibberellin,dihydrozeatin riboside,pelargonidin,apigenin and cyanidin changed significantly in phytoplasma-infected seedlings compared with the healthy ones,suggesting that variations in concentrations of these metabolites might be related to PaWB.[Conclusion] Variation in metabolite concentrations among the healthy and phytoplasma-infected seedlings can help reveal the candidate metabolites and inherent pathways that are potentially involved in PaWB.This study will provide new insight into the role of metabolites in Paulownia and other trees in response to phytoplasma infection.关键词
毛泡桐/代谢组/丛枝病/植物激素/花青素Key words
Paulownia tomentosa/metabolomics/witches' broom/plant hormones/anthocyanin分类
农业科技引用本文复制引用
曹亚兵,翟晓巧,邓敏捷,赵振利,范国强..泡桐丛枝病发生与代谢组变化的关系[J].林业科学,2017,53(6):85-93,9.基金项目
National Natural Science Foundation of China (U1204309) （U1204309）
Outstanding Talents Project of Henan Province (122101110700) （122101110700）
Natural Science Foundation of Henan Province of China (162300410158) （162300410158）
Outstanding Talents Project of Henan Province (174200510001). （174200510001）
