林业科学2017，Vol.53Issue(6)：105-110,6.DOI:10.11707/j.1001-7488.20170612
松材线虫CYP450基因与松树蒎烯类物质代谢的相关性
Relationship between the Cytochrome P450 Gene of Pine Wood Nematode and the Accumulation of Pine Pinene
摘要
Abstract
[Objective] By comparing the expression pattern of cytochrome P450 (CYP450) genes of Pine Wood Nematode (PWN) (Bursaphelenchus xylophilus)and the accumulation of pine terpene,the relationship between the CYP450 genes of PWN and the metabolism of pine pinene was analyzed to provide fundamental information for PWN pathogenic mechanisms study.[Method]Five-years Pinus massoniana were inoculated with PWN.Real-time Quantitative PCR was conducted to detect expression patterns of apin gene of pine trees and CYP450 family genes of PWN.Terpene metabolism in infected P.massoniana was detected by Gas Chromatography.[Result]The expression of pinene synthase genes were dramatically induced at 6 dpi (days post inoculation) and 21 dpi.Both α-pinene and β-pinene were accumulated with two peaks at 9dpi and 27dpi,respectively.Correspondingly,the expression of PWN CYP-33C9 dramatically increased at 12dpi and 15dpi,and the expression of CYP-33C4 were highest at 21dpi.[Conclusion]The pine tree pinene synthase gene was highly induced by PWN inoculation which resulted in sharp accumulation of the α-pinene and β-pinene.In responding to the accumulation of pine terpene,the expression of PWN CYP450 genes dramatically increased.Therefore,according to the temporal correlation between the pinene metabolism and the PWN CYP450 genes expression we inferred that the PWN CYP450 genes might participate in the terpene metabolism and might be one of the pathogenic genes in the PWN pathogenic process.关键词
松材线虫/蒎烯代谢/细胞色素P450/致病机制Key words
pine wood nematode/terpene metabolism/cytochrome P450/pathological mechanisms分类
农业科技引用本文复制引用
王璇,理永霞,刘振宇,吕全,贾秀贞,张星耀..松材线虫CYP450基因与松树蒎烯类物质代谢的相关性[J].林业科学,2017,53(6):105-110,6.基金项目
浙江省省院合作林业科技项目(2014SY17) （2014SY17）
林业公益性行业科研专项经费(201204501). （201204501）
