国际眼科杂志2017，Vol.17Issue(8)：1387-1393,7.DOI:10.3980/j.issn.1672-5123.2017.8.01
CTGF siRNA转染对体外培养人晶状体上皮细胞株B3转分化的抑制作用
Transfection of CTGF siRNA inhibits transdifferentiation in human lens epithelium cell line B3 in vitro
摘要
Abstract
AIM: To investigate the expression of connective tissue growth factor (CTGF) and α-SMA in human lens epithelium cell (HLEC) line B3 after transfection by liposome-coated siRNA targeting CTGF.METHODS: HLECs were transfected with small interfering RNA (siRNA) targeting CTGF,labeled with 5`-fluorescein isothiocyanate (5`-FITC) and coated with lipofectamine.The transfection ratio was evaluated via fluorescence intensity.Cell counting kit-8 (CCK-8) assay was performed to assess cytoviability of both non-transfected and transfected HLECs.Quantitative RT-PCR,cell immunochemistry and Western blot analysis were conducted to detect the expression changes of CTGF and α-SMA after transfection.RESULTS: A highly effective transfection ratio was observed in siRNA co-transfected with lipofectamine.The transfection ratio reached 95% at 24h.The proliferation of HLECs was inhibited by siRNA after 72h transfection.The expression of CTGF and α-SMA significantly decreased in HLECs after transfected by CTGF siRNA for 24h.This effect was not found in negative control siRNA.CONCLUSIONS: SiRNA targeting CTGF decreased CTGF and α-SMA expression in HLECs,which is a potential therapeutic strategy for posterior capsular opacification.关键词
结缔组织生长因子/小干扰RNA/脂质体/转分化Key words
connective tissue growth factor/small interfering RNA/lipofectamine/transdifferentiation引用本文复制引用
庄华,郑凝旋,巫婧,徐巍,胡建章,谢茂松,郭建,徐国兴..CTGF siRNA转染对体外培养人晶状体上皮细胞株B3转分化的抑制作用[J].国际眼科杂志,2017,17(8):1387-1393,7.基金项目
福建省卫生系统中青年骨干人才培养项目(2014-ZQN-ZD-16) （2014-ZQN-ZD-16）
Young and Middle Aged Key Personnel Training Project of Fujian Provincial Health and Family Planning Commission (2014-ZQN-ZD-16) （2014-ZQN-ZD-16）
