可再生能源2017，Vol.35Issue(7)：949-956,8.
高效液相色谱法同时测定油菜秸秆水解液中的糠醛和5-羟甲基糠醛
Simultaneous determination of furfural and 5-hydroxymethylfurfural in the hydrolysate of rape straw by high performance liquid chromatography
摘要
Abstract
To analyze the hydrolysis process, separate and make use of the components in straw hydrolysate, it is important to determine them. In this work, furfural (F) and 5 -hydroxymethylfurfural (5-HMF) which are the degradation products of monosaccharides in biomass hydrolysate were detected by high performance liquid chromatography (HPLC). The samples were separated by AlltimaTM C18 column at 30℃. The mobile phase was acetonitrile and water (with 0.1%acetic acid) under a fixed volume ratio of 10:90, and the flow rate was 1.0 mL/min. They were detected by ultraviolet (UV) detector at 285 nm. Excellent linearity can be obtained (R2:0.9998 for F and 0.9999 for 5-HMF). The average recovery values were 99.8%and 100.3%respectively, and the relative standard deviation percentage (R SD ) was less than 1%. This method can achieve good precision, high accuracy and good reproducibility, and provide an accurate, sensitive and rapid analysis method for F and 5-HMF in straw hydrolysate.关键词
高效液相色谱/秸秆水解液/糠醛/5-羟甲基糠醛Key words
high performance liquid chromatography/straw hydrolysate/furfural/5-hydroxyme-thylfurfural分类
能源科技引用本文复制引用
冯广荣,鲁厚芳,梁斌,刘颖颖..高效液相色谱法同时测定油菜秸秆水解液中的糠醛和5-羟甲基糠醛[J].可再生能源,2017,35(7):949-956,8.基金项目
国家自然科学基金项目(21336008). （21336008）
