南京医科大学学报（自然科学版）2017，Vol.37Issue(6)：669-673,702,6.DOI:10.7655/NYDXBNS20170604
长链非编码RNA PCAT4在前列腺癌中的表达及意义
Expression and clinical significance of long non-coding RNA PCAT4 in prostate cancer
摘要
Abstract
Objective:To investigate the expression of long non-coding RNA (lncRNA) prostate cancer associated transcript 4 (PCAT4) and its clinical significance in prostate cancer.Methods:Real-time-PCR method was used to determine the relative expression of PCAT4 in 48 cancer samples and corresponding paracancerous tissues from patients with prostate cancer,as well as prostate cancer cells PC3,22RV1 and normal myofibroblast stromal cells WPMY-1.The relationship between the expression level of PCAT4 and the clinicopathological characteristics of patients with prostate cancer was analyzed.We detected the effects of PCAT4 on the cell proliferation and migration of prostate cancer cells by RNA interference,CCK-8 assay,and migration experiment.Results:The relative expression levels of PCAT4 in the prostate cancer tissues and adjacent tissues were 6.15±1.58 and 1.77±0.65,respectively (P＜ 0.001),while the relative expression levels of PCAT4 in the prostate cancer cell line PC3 and 22RV1 were higher than that in WPMY-1.The high expression of PCAT4 in the prostate cancer tissues was found in 30 cases of 48 cases,and 18 cases were low expression.The relative expression level of PCAT4 in prostate cancer tissues was associated with Gleason score and T stage (both P＜ 0.05),and was not associated with age,tumor size,lymph node metastasis and multiple lesions (all P＞0.05).In addition,the siRNA-mediated knockdown of PCAT4 in PC3 and 22RV1 cell lines significantly decreased the ability of proliferation and migration (all P＜ 0.05).Conclusion:The relative expression level of PCAT4 was elevated in prostate cancer.PCAT4 may promote the carcinogenesis and development of prostate cancer by affecting the proliferation and migration of prostate cancer cells,which can be used as a potential novel target for the diagnosis and treatment.关键词
前列腺癌/长链非编码RNA/PCAT4Key words
prostate cancer/long non-coding RNA/PCAT4分类
医药卫生引用本文复制引用
张成,秦超,吴杰,成功,华立新..长链非编码RNA PCAT4在前列腺癌中的表达及意义[J].南京医科大学学报（自然科学版）,2017,37(6):669-673,702,6.基金项目
江苏省自然科学基金(BK20141495) （BK20141495）
