青岛大学医学院学报2017，Vol.53Issue(2)：127-129,132,4.DOI:10.13361/j.qdyxy.201702001
淫羊藿苷对AD小鼠炎症反应和学习记忆能力影响
EFFECT OF ICARIIN ON INFLAMMATORY RESPONSE AND LEARNING AND MEMORY ABILITIES IN MICE WITH ALZHEIMER'S DISEASE
摘要
Abstract
Objective To investigate the effect of icariin (ICA) in inhibiting inflammatory resoponse and improving learning and memory abilities in ICR mice with lipopolysaccharide (LPS) induced Alzheimer's disease (AD).Methods A total of 18 ovariectomized mice were randomly divided into control group,LPS group,and ICA+ LPS group,with 6 mice in each group.The mice in the control group were given dimethyl sulfoxide by gavage.The mice in the LPS group were given injection of LPS in the lateral ventricle to establish a mouse model of AD,followed by dimethyl sulfoxide by gavage.The mice in the ICA+LPS group were given injection of LPS in the lateral ventricle and then continuously given ICA by gavage.The course of treatment was 12 days.The water maze test was used to test learning and memory abilities,real-time reverse transcription-polymerase chain reaction was used to measure the mRNA expression of inducible nitric oxide synthase (iNOS) and tumor necrosis factor-α (TNF α).Results Compared with the control group,the LPS group had significant reductions in the proportion of time spent in the target quadrant and number of platform crossings and significant increases in the mRNA expression of iNOS and TNF-α in the hippocam pus.Compared with the LPS group,the ICA+LPS group had significantly less injury induced by LPS,significant increases in the proportion of time spent in the target quadrant and number of platform crossings,and significant reductions in the mRNA expression of iNOS and TNF-α (F=9.214-57.090,q-4.114-13.400,P＜0.05).Conclusion In ICR mice with LPS-induced AD and inflammation,ICA can inhibit the mRNA expression of iNOS and TNF-α in the hippocampus and improve their learning and memory abilities.关键词
淫羊藿苷/炎症/脂多糖类/阿尔茨海默病/小鼠Key words
icariin/inflammation/lipopolysaccharides/Alzheimer disease/mice分类
生物科学引用本文复制引用
赵霞,都中蕊,陈文芳..淫羊藿苷对AD小鼠炎症反应和学习记忆能力影响[J].青岛大学医学院学报,2017,53(2):127-129,132,4.基金项目
国家自然科学基金资助项目(31271150,30971004) （31271150,30971004）
