青岛大学医学院学报2017，Vol.53Issue(2)：130-132,3.DOI:10.13361/j.qdyxy.201702002
枸橼酸铁铵与α-syn对C6细胞释放炎性因子影响
EFFECT OF FERRIC AMMONIUM CITRATE ON RELEASE OF PRO-INFLAMMATORY CYTOKINES IN C6 GLIOMA CELLS EXPOSED TO EXTRACELLULAR A-SYNUCLEIN
摘要
Abstract
Objective To examine the effect of ferric ammonium citrate (FAC) on the release of pro-inflammatory cytokines by C6 glioma cells exposed to extracellular α-synuclein (α-syn).Methods C6 cells were stimulated with α-syn,FAC,α-syn+ FAC,or serum-free medium (control).The mRNA expression of intracellular interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) was measured using real-time PCR.Results The IL 1β and TNF-α expression in the α-syn group was significantly higher than that in the control group (F=5.43-67.19,q=4.07-16.25,P＜0.05).On the other hand,while there were no significant differences in IL 1β and TNF-α expression between the 10 μmol/L FAC group and the control group (P＞0.05),IL 1β and TNF-α expression was significantly enhanced in the 100 μmol/L FAC group compared with the control group (q =14.48 4.14,P ＜0.05).However,there were no significant differences in IL-1β and TNF-α expression between the α-syn+ FAC group and α-syn group (P＞0.05).Conclusion Extracellular α-syn can stimulate C6 cells to release pro-inflammatory cytokines,which is not affected by the increase in iron levels.关键词
铁化合物/α突触核蛋白/星形细胞/白细胞介素1β/肿瘤坏死因子αKey words
iron compound/alpha-synuclein/astrocyte/interleukin-1β/tumor necrosis factor-α分类
医药卫生引用本文复制引用
郭新丽,宋宁,姜宏,谢俊霞..枸橼酸铁铵与α-syn对C6细胞释放炎性因子影响[J].青岛大学医学院学报,2017,53(2):130-132,3.基金项目
国家自然科学基金项目(81430024)和山东省自然科学基金项目(ZR2014JL022) （81430024）
