青岛大学医学院学报2017，Vol.53Issue(2)：133-135,139,4.DOI:10.13361/j.qdyxy.201702003
Rg1对胶质细胞iNOS基因表达的抑制作用及RU486对其影响
INHIBITORY EFFECT OF RGI ON EXPRESSION OF INDUCIBLE NITRIC OXIDE SYNTHASE IN GLIOCYTES AND THE INFLUENCE OF RU486 ON RG1 ACTION
摘要
Abstract
Objective To investigate the inhibitory effect of ginsenoside Rg1 on lipopolysaccharide (LPS)-induced inflammatory response in primary cultured astrocytes and BV2 microglial cells and the influence of glucocorticoid receptor (GR) antagonist RU486.Methods Primary astrocytes and BV2 microglial cells were cultured and randomly divided into control group,LPS group,Rg1+LPS group,and RU486+Rg1+LPS group.No treatment was given to the cells in the control group;the cells in the other three groups were treated with RU486 (1 μmol/L) for 1 h in the presence or absence of Rg1 pretreatment,followed by 1 mg/L LPS for 6 h.Real-time reverse transcription-polymerase chain reaction was used to measure the mRNA expression of inducible nitric oxide synthase (iNOS).Results Compared with the control group,the LPS group had a significant increase in the mRNA expression ofiNOS inastrocytes (F=82.69,q=21.53,P＜0.01) and BV2 microglial cells (F=18.73,q=8.974,P＜0.01).Compared with the LPS group,the Rg1 + LPS group showed significant inhibition of the inductive effect of LPS on iNOS in astrocytes and BV2 microglial cells (q =9.799,6.640;P ＜ 0.01).There was no significant difference in the mRNA expression of iNOS between the RU486+Rg1+LPS group and the Rg1+LPS group (P＞0.05).Conclusion Ginsenoside Rg1 can inhibit LPS-induced mRNA expression of iNOS in astrocytes and BV2 microglial cells.This effect cannot be blocked by the GR antagonist RU486.关键词
人参皂苷Rg1/受体,糖皮质激素/诱导型一氧化氮合成酶/星形胶质细胞/小胶质细胞Key words
ginsenoside Rg1/receptors, glucocorticoid/inducible nitric oxide synthase/astrocyte/microglia分类
生物科学引用本文复制引用
张学杰,任晓璠,陈文芳..Rg1对胶质细胞iNOS基因表达的抑制作用及RU486对其影响[J].青岛大学医学院学报,2017,53(2):133-135,139,4.基金项目
国家自然科学基金资助项目(31271150,30971004) （31271150,30971004）
