青岛大学医学院学报2017，Vol.53Issue(2)：136-139,4.DOI:10.13361/j.qdyxy.201702004
植酸对结直肠癌模型大鼠Wnt信号通路的影响
EFFECT OF PHYTIC ACID ON THE WNT SIGNALING PATHWAY IN A RAT MODEL OF COLORECTAL CANCER
摘要
Abstract
Objective To determine the mechanism ot action of phytic acid in regulating colorectal cancer cell cycle via the Wnt signaling pathway in a rat model of colorectal cancer.Methods Sixty Wistar rats were randomly assigned to control group,model group,low-dose phytic acid group,medium-dose phytic acid group,and high-dose phytic acid group.The control group and model group were given saline daily by gavage,while the three phytic acid groups were given sodium phytate daily at doses of 0.25,0.50,and 1.00 g/kg body weight,respectively.After 4 weeks of phytic acid administration,a rat model of colorectal cancer was established in the model group,low-dose phytic acid group,medium-dose phytic acid group,and high-dose phytic acid group.Changes in the body weight of rats in the five groups were recorded.Upon being sacrificed,tumor incidence in the five groups was analyzed based on the expression of Wnt signaling pathway-related proteins pGSK-3β,active β-catenin,and CyclinD1 in colorectal cancer or control tissues measured by Western Blot.Cell cycle in these tissues was also examined using flow cytometry.Results Compared with the model group,rats in the phytic acid-treated groups had less body weight loss,and the colorectal cancer incidence was also reduced in the medium-and high-dose phytic acid groups.The protein expression of pGSK-3β,active β-catenin,and CyclinD1 was progressively reduced with phytic acid administration in a dose-dependent manner (F =5.400-13.534,P＜0.05).Cell reduction in the S phase and cell arrest in the G1 phase were also observed in the tumor cells after phytic acid treatment (F=4.268,28.467;P＜0.05).Conclusion Phytic acid can inhibit the expression of Wnt signaling pathway-related proteins and regulate tumor cell cycle to reduce the incidence of colorectal cancer in a dose-dependent manner.关键词
植酸/结直肠肿瘤/Wnt信号通路/细胞周期/大鼠,WistarKey words
phytic acid/colorectal cancer/Wnt signaling pathway/cell cycle/rats, Wistar分类
医药卫生引用本文复制引用
于文洋,刘翠萍,陈沉,程立雪,刘畅,宋扬..植酸对结直肠癌模型大鼠Wnt信号通路的影响[J].青岛大学医学院学报,2017,53(2):136-139,4.基金项目
国家自然科学基金资助项目(81373001) （81373001）
