青岛大学医学院学报2017，Vol.53Issue(2)：150-152,156,4.DOI:10.13361/j.qdyxy.201702008
5-氮杂胞苷对多发性骨髓瘤MM1R及RPMI8226细胞增殖的影响
THE EFFECT OF 5-AZA-CYTIDINE ON THE PROLIFERATION OF MULTIPLE MYELOMA CELL LINE MM1R AND RPMI8226
樊馥荔 1王伟 1李广伦 1吴颖 1鞠小妍 1王颜刚2
作者信息
- 1. 青岛大学附属医院血液科,山东青岛266003
- 2. 青岛大学附属医院内分泌科,山东青岛266003
- 折叠
摘要
Abstract
Objective To investigate the effect of 5-aza-cytidine on the proliferation of multiple myeloma cell line MM1R and RPMI8226.Methods Human multiple myeloma cell line MM1R and RPMI8226 were cultured to logarithmic growth phase and MM1R resistance was verified.Different concentrations of 5-aza-cytidine were added to co-culture with MM1R and RPMI8226.The proliferation activity of MM1R and RPMI8226 at different time was detected using CCK8,the expression of IFN-γin MM1R and RPMI8226 was measured applying ELISA.Results At the same action time,the proliferative activity of MM1R and RPMI8226 decreased with the increase of concentration of 5-aza-cytidine (F =78.68,63.12;P ＜0.05).Under the same concentration of 5-aza-cytidine,the proliferation of MM1R and RPMI8226 decreased along with the time extending (F =112.12,99.87;P＜0.05).When the action time and the concentration of 5-aza-cytidine were the same,the cell proliferation and inhibition rate (CPIR) of MM1R was higher than that of RPMI8226.When these two cells were treated with different concentrations of 5-aza-cytidine,the IFN-γ expression in the two cells increased with the increase of 5-aza-cytidine concentration (F=46.78,58.67;P＜0.05).Conclusion The 5-aza-cytidine has inhibition action on proliferation of MM1R and RPMI8226 cells in human multiple myeloma,and the inhibitory effect on MM1R was stronger than that on RPMI8226,and at the same time,5-aza-cytidine has a role in promoting the secretion of IFN-γ.关键词
胞苷/多发性骨髓瘤/细胞增殖/干扰素γKey words
cytidine/multiple myeloma/cell proliferation/interferon-gamma分类
医药卫生引用本文复制引用
樊馥荔,王伟,李广伦,吴颖,鞠小妍,王颜刚..5-氮杂胞苷对多发性骨髓瘤MM1R及RPMI8226细胞增殖的影响[J].青岛大学医学院学报,2017,53(2):150-152,156,4.
