青岛大学医学院学报2017，Vol.53Issue(2)：153-156,4.DOI:10.13361/j.qdyxy.201702009
脐带间充质干细胞对衰老心肌细胞炎症因子表达影响
EFFECT OF UMBILICAL CORD MESENCHYMAL STEM CELLS ON EXPRESSION OF INFLAMMATORY FACTORS IN SENESCENT CARDIOMYOCYTES
摘要
Abstract
Objective To investigate the effect of human umbilical cord mesenchymal stem cells (hUC-MSCs) on the expression of inflammatory factors in senescent cardiomyocytes induced by hydrogen peroxide (H2 O2).Methods Senescence of H9c2 cardiomyocytes was induced by 100 μmol/L H2 O2,and DAPI fluorescent staining was used to measure the proportion of cells with senescence-associated heterochromatin foci (SAHF),in order to determine whether the senescence model was successfully established.Transwell chambers were used to establish a co-culture system of H9c2 cardiomyocytes and hUC-MSCs;DAPI fluorescent staining was used to investigate the influence of hUC-MSC culture on the proportion of SAHF-positive H9c2 cardiomyocytes,and ELISA was used to measure the expression of tumor necrosis factor-α (TNF α),interleukin-1β (IL-1β),and interleukin-10 (IL-10) in supernatant.Results DAPI fluorescent staining showed that compared with the control group,the H2O2 group had a significant increase in the proportion of SAHF-positive cells (F=22.658,q=9.562,P＜0.05),suggesting that the model of cardiomyocyte senescence was established successfully.Compared with the H2 O2 group,the H2 O2 +hUC-MSCs group had a significant reduction in the proportion of SAHF positive cells (q =7.092,P＜0.05).ELISA results suggested that compared with the H2 O2 group,the H 2O2 +hUC-MSCs group had significant reductions in the expression of proinflammatory factors TNF-α (F =7.338,q=4.334,P＜0.05) and IL-1β (F=13.965,q=6.144,P＜0.05) in supernatant,as well as a significant increase in the expression of the anti-inflammatory factor IL-10 (F=17.995,q=6.405,P＜0.05).Conclusion hUC-MSCs have a protective effect against the senescence of H9c2 cardiomyocytes induced by H2 O2,possibly by reducing the expression of proinflammatory factors and increasing the expression of anti-inflammatory factors.关键词
间质干细胞/脐带/细胞衰老/肌细胞,心脏/肿瘤坏死因子α/白细胞介素类Key words
mesenchymal stem cells/umbilical cord/aging/myocytes, cardiac/tumor necrosis factor-alpha/interleukins分类
医药卫生引用本文复制引用
张蕾,冯文静,高宏,胡松,张敏,毛拥军..脐带间充质干细胞对衰老心肌细胞炎症因子表达影响[J].青岛大学医学院学报,2017,53(2):153-156,4.基金项目
国家自然科学基金资助项目(No.31640050 （）
No.31571829) （）
山东省自然科学基金青年基金项目(ZR2016HQ23) （ZR2016HQ23）
