青岛大学医学院学报2017，Vol.53Issue(2)：198-202,5.DOI:10.13361/j.qdyxy.201702022
乳酸菌对铜暴露大鼠肝功能及氧化应激的影响
EFFECT OF LACTOBACILLUS ON LIVER FUNCTION AND OXIDATIVE STRESS IN RATS EXPOSED TO COPPER
摘要
Abstract
Objective To investigate the influence of low dose Copper exposure on liver function and oxidative stress in rats,as well as the effect of Lactobacillus (LAB) intervention on liver impairment and changes in oxidative stress level caused by Copper exposure.Methods A total of 64 Wistar rats were randomly divided into high-Copper group (14.000 mg/kg),mediumCopper group (3.500 mg/kg),low-Copper group (0.875 mg/kg),high-Copper+LAB group (14.000 mg/kg Copper+LAB),medium-Copper+ LAB group (3.500 mg/kg Copper+ LAB),low-Copper + LAB group (0.875 mg/kg Copper + LAB),LAB group,and blank control group,with 8 rats in each group.The rats were treated by gavage for 30 d.After the treatment ended,pathological changes of the liver were observed,liver,spleen,and thymus indices were calculated,and the levels of liver function parameters,alanine aminotransferase (ALT),aspartate aminotransferase (AST),and albumin (ALB),and oxidative stress indices,superoxide dismutase (SOD) and malondialdehyde (MDA),were measured.Results Pathological examination showed that compared with the high-Copper group,the high-Copper+LAB group had significant improvements in liver injury.The results of biochemical analysis showed that compared with the blank control group,the high-,medium-,and low-Copper groups had significant increases in AST and ALT and a significant reduction in ALB (F-8.712-19.134,P ＜ 0.05).Compared with the high-Copper group or the medium Copper group,the high-Copper+ LAB group or the medium-Copper+ LAB group had significant reductions in AST and ALT and a significant increase in ALB (t =2.257-10.199,P ＜0.05).Compared with the low-Copper group,the lowCopper+LAB group had a significant reduction in ALT and a significant increase in ALB (t =3.161,4.032;P＜0.05).The analysis of oxidative stress indices showed that compared with the blank control group,the high-and medium-Copper groups had a significant reduction in SOD activity and a significant increase in the content of MDA (F =28.900,119.900;P ＜0.05).Compared with the high Copper group or the medium-Copper group,the high-Copper+LAB group or the medium-Copper+LAB group had a significant increase in SOD level and a significant reduction in the content of MDA (t=2.069-5.214,P＜0.05).Conclusion A relatively low dose of Copper can affect liver function and oxidative stress response in rats,and LAB intervention helps to improve liver function and oxidative stress in high-,medium-,and low dose Copper sulfate groups.关键词
乳酸菌/铜/肝功能/氧化应激Key words
lactic acid bacteria/liver function/Copper/oxidative stress分类
医药卫生引用本文复制引用
刘云才,张娟,焦聪聪,崔莲花,蒋梦会,朴金梅..乳酸菌对铜暴露大鼠肝功能及氧化应激的影响[J].青岛大学医学院学报,2017,53(2):198-202,5.基金项目
青岛市博士后应用研究项目(2015166) （2015166）
