热带作物学报2017，Vol.38Issue(6)：1088-1093,6.DOI:10.3969/j.issn.1000-2561.2017.06.017
基于高通量测序技术分析石蝉草内生细菌多样性
Endophytic Bacterial Diversity of Peperomia dindygulensis Miq.Estimated via High Throughput Sequencing
摘要
Abstract
In this study,the species and diversity of endophytic bacteria in the root,stem and leaf of Peperomia dindygulensis Miq.were comprehensively investigated by highthroughput sequencing technology based on Illumina Miseq platform.The results showed that the number of OTUs were 167 in total which belonged to 11 phyla respectively as Proteobacteria,Ignavibacteriae,Gemmatimonadetes,Fusobacteria,Firmicutes,Deinococcus-Thermus,Chloroflexi,Bacteroidetes,Actinobacteria and Acidobacteria.There were differences in the community composition and diversity of endophytic bacteria from different samples,but the dominant groups of 3 samples were Proteobacteria,accounting for 62.48％-93.24％.In this research,it is showed that Bacillus and Myxococcus on genus level were specifically located in the leaf and root,respectively.These may have some correlation with preferable anti-inflammation and anti-tumor effects.关键词
石蝉草/内生细菌/高通量测序/多样性Key words
Peperomia dindygulensis Miq./endophytic bacteria/high-throughput sequencing/diversity分类
农业科技引用本文复制引用
靳松,陈泽斌,李育川,夏体渊,赵凤,任稹..基于高通量测序技术分析石蝉草内生细菌多样性[J].热带作物学报,2017,38(6):1088-1093,6.基金项目
云南省教育厅科学研究基金项目(No.2015Y393) （No.2015Y393）
昆明学院科学研究项目(No.XJZD1605) （No.XJZD1605）
云南省高校特色生物资源开放与利用重点实验室开放基金项目(No.GXKJ201622) （No.GXKJ201622）
昆明学院大学生科研项目(No.XJD16082). （No.XJD16082）
