热带作物学报2017，Vol.38Issue(6)：1094-1100,7.DOI:10.3969/j.issn.1000-2561.2017.06.018
剑麻斑马纹病菌EST-SSR标记开发与评价
Development and Evaluation of EST-SSR Marker for Phytophora nicotianae var.parasitica Causing Zebra Spot Disease
摘要
Abstract
Zebra spot disease caused by Phytophora nicotianae var.parasitica (or Phytophora parasitica var.nicotianae) is the most serious and devastating fungi disease in sisal.In the present study,a total of 5 519 non-redundant P.nicotianae were generated from the 10 525 publicly available EST sequences by CAP3 software,which used to mine potential microsatellites by microsatellite identification tool (MISA).A total of 199 SRR was found in P.nicotianae expressed-squence tags data.Of the 199 SSR,the most abundant SSR was the tri-nucleotide type,with the SSR numbers being 55,accounting for 27.6％,followed by the di-nucleotide and mono-nucleotide type,with the SSR numbers being 51 and 47,accounting for 25.6％ and 23.6％.Among the di-nucleotide repeats,AG/CT was the most motifs and accounted for 76.5％.The AAG/CTT and AGC/CTG was the most motifs in tri-nucleotide,being 15 and 11,accounting for 27.3％ and 20.0％,respectively.Further screening to the number of repetitions and motifs relatively large number of SSR among the 199 SRR,which the right position to develop molecular markers,and a total of 22 primers were developed.Among the 22 primer pairs designed and synthesized,there were only 9 primer pairs that amplified characteristic SSR bands in genomic DNA of 18 tested isolates of P.nicotiana from sisal,thus the transferability was 40.9％.The results showed that it is feasible for molecular markers using this method to develop the sisal zebra germs,only a transfer rate of problems.关键词
剑麻斑马纹病菌/表达序列标签(EST)/SSR/分子标记Key words
Phytophora nicotianae var.parasitica / Phytophora parasitica var.nicotianae/expressed sequence tag (EST)/simple sequence repeat (SSR)/molecular marker分类
数理科学引用本文复制引用
吴伟怀,贺春萍,易克贤,汪涵,汪全伟,习金根,郑金龙,梁艳琼,李锐,黄兴,杨蕊馨..剑麻斑马纹病菌EST-SSR标记开发与评价[J].热带作物学报,2017,38(6):1094-1100,7.基金项目
国家麻类产业技术体系建设专项资金资助(No.CARS-19) （No.CARS-19）
国家自然科学基金(No.31371679) （No.31371679）
农业部热作病虫害疫情监测与防治项目(No.16RZBC-14). （No.16RZBC-14）
