水产学报2017，Vol.41Issue(7)：993-1005,13.DOI:10.11964/jfc.20160310332
金钱鱼促性腺激素受体基因克隆及其在性腺发育不同时期mRNA表达水平的分析
Gene cloning of gonadotropic hormone receptors and their mRNA expressions at different development stages of the gonad inScatophagus argus
摘要
Abstract
In the present study, follicle-stimulating hormone receptor (FSHR) and luteinizing hormone receptor(LHR) genes were cloned by using reverse transcription-polymerase chain reaction (RT-PCR) and rapidamplification of cDNA 5′ and 3′ ends (RACE) in Scatophagus argus. The full-length of FSHR cDNA was 2538bp, encoding a 702 amino acid protein and the full-length of LHR cDNA was 3315 bp, encoding a 722 amino acidprotein. Both of them contain seven TM helix domains, a feature of glycoprotein hormone receptor (GHRs) family.Multiple sequence alignment shows that S. argus GtHRs have the highest homology with that of Dicentrarchuslabrax. mRNA expression patterns showed that a high expression level of FSHR was detected at stage Ⅰ in ovary,followed by an obvious decrease at stage Ⅱ, Ⅲ and IV. In the testis, the expression of FSHR gene increasedgradually during early stages (stage Ⅰ, Ⅱ and Ⅲ), and apparently reduced at stage Ⅳ. Our results indicates thataccording to H.E staining, sperm maturation started at stage Ⅲ, implying FSHR played an important role in earlystages of the gonadal development, and LHR is vital for sperms/oocytes maturation and release as well as sperms.关键词
金钱鱼/卵泡刺激素受体/促黄体生成素受体/序列分析/基因表达Key words
Scatophagus argus/FSHR/LHR/sequence analysis/gene expression分类
生物科学引用本文复制引用
张逛,王武,段征宇,桂朗,苏冒亮,牟幸江,张俊彬..金钱鱼促性腺激素受体基因克隆及其在性腺发育不同时期mRNA表达水平的分析[J].水产学报,2017,41(7):993-1005,13.基金项目
国家自然科学基金(31272662) （31272662）
上海市教委科研创新项目(14YZ117)National Natural Science Foundation of China (31272662) （14YZ117）
Innovation Program of Shanghai Municipal Education Commission (14YZ117) （14YZ117）
