水产学报2017，Vol.41Issue(7)：1054-1063,10.DOI:10.11964/jfc.20150810044
文蛤粪卟啉原Ⅲ氧化酶基因克隆及与壳色性状的相关性分析
Gene cloning of CPOX and correlation analysis of shell colors in Meretrix meretrix
摘要
Abstract
Coproporphyrinogen-Ⅲ oxidase (CPOX) is a key enzyme in the synthesis of porphyrins, which plays an important role in the formation of pigment. cDNA of Meretrix meretrix CPOX (MmCPOX) was cloned by SMART RACE techniques, then the bioinformatics and expression profiles in different tissues and developmental stages were analyzed. The results indicated that the full length cDNA of MmCPOX gene was 1490 bp, containing a 1173 bp opening reading fame(ORF) encoding 390 amino acids. The molecular weight of MmCPOX was 12.04 ku and pI was 5.05. It was also predicted that protein MmCPOX had a transmembrance region and a Coprogen-oxidas region. Comparisons of amino acid sequence, MmCPOX was highly homologous withAplysia californica and shares 64.5% similarity. The result of qRT-PCR showed that MmCPOX gene was expressed in all stages, and significantly increased from D-shaped larva stage (P<0.01).MmCPOX was expressed in all seven tissues, in which the expressions in mantle and blood were significantly higher than those of other tissues (P<0.01), indicating that it was related to synthesis of hematoporphyrin and formation of shell porphyrin. Considering different shell colors, the relative expressions of mantle in red color, dark fringe and thin checkered shells, were significantly different from the black and white shells (P<0.05), suggesting that MmCPOX gene was responsible for the formation of red and brown shell color.关键词
文蛤/粪卟啉原Ⅲ氧化酶/壳色/克隆与表达Key words
Meretrix meretrix/CPOX/shell color/cloning and expression分类
生物科学引用本文复制引用
詹艳玲,董迎辉,何琳,阮文斌,高晓艳,林志华..文蛤粪卟啉原Ⅲ氧化酶基因克隆及与壳色性状的相关性分析[J].水产学报,2017,41(7):1054-1063,10.基金项目
国家现代贝类产业技术体系专项(CARS-48) （CARS-48）
浙江省自然科学基金(Y16C190015) （Y16C190015）
江苏省苏北科技专项(BN2015059) （BN2015059）
宁波市科技计划项目(2016C70002) Modern Agro-industry Technology Research System (CARS-48) （2016C70002）
Zhejiang Provincial Natural Science Foundation (Y16C190015) （Y16C190015）
Science and Technology Project of Jiangsu Province (BN2015059) （BN2015059）
Ningbo Project of Agricultural Science and Technology (2016C70002) （2016C70002）
