水产学报2017，Vol.41Issue(7)：1073-1082,10.DOI:10.11964/jfc.20151210204
辽宁沿海日本海马线粒体控制区序列变异及其在海龙科鱼类系统分析中的应用
Sequence variation of mtDNA control region inHippocampus japonicus inhabiting Liaoning coast and its applicability as a marker for phylogenetic analysis
摘要
Abstract
To study the germplasm resources and genetic diversity of the wild population of Hippocampus japonicus, the mitochondrial DNA control region fragments were obtained from wild populations by PCR amplification. The homologous sequences of other Syngnathidae fishes from Gen Bank were also included in this study. PCR amplification products of 557–558 bp CR fragments were obtained, and the average contents of A, T, G and C were 34.3%, 29.7%, 14.1%, 21.9%, respectively. A total of 16 polymorphic nucleotide sites were detected, which defined 16 haplotypes. The variation level was low asH was 0.70±0.02 andπ was 0.0032±0.0021. With the aid of the homologous sequences retrieved from Gen Bank, phylogenetic trees were constructed based on neibour-joining (NJ), maximum-likelihood (ML) and Bayesian inference (BI) methods to build phylogenetic relationships within the Syngnathidae fishes. The phylogenic analysis showed that the topology of phylogenetic trees were almost consistent with each other, although the NJ tree and the ML/BI tree were somewhat different. The phylogenic analyses of CR gene sequences were also consistent with morphological taxonomy. Based on the results, several conclusions were drawn as follows: (1) CR is an appropriate marker for the seahorse species identification and population genetic analysis; (2) CR is highly divergent among different Syngnathidae fishes and could be an appropriate marker for molecular systematic studies. This study is expected to provide important information for the protection and utilization of H. japonicus resources in China.关键词
日本海马/海龙科/线粒体DNA/控制区序列/序列变异/系统分析Key words
Hippocampus japonicus/Syngnathidae/mtDNA/control region/sequence variation/phylogenetic analysis分类
生物科学引用本文复制引用
李玉龙,王彬,王爱勇,于旭光,李轶平,韩振华,付杰,董婧..辽宁沿海日本海马线粒体控制区序列变异及其在海龙科鱼类系统分析中的应用[J].水产学报,2017,41(7):1073-1082,10.基金项目
海洋公益性行业科研专项(201405010) （201405010）
辽宁省海洋与渔业科研项目(201401)Public Science and Technology Research Funds Projects of Ocean (201405010) （201401）
Liaoning Ocean and Fishery Science Research Funds Projects (201401) （201401）
