水产学报2017，Vol.41Issue(7)：1083-1095,13.DOI:10.11964/jfc.20160410360
罗氏沼虾种群SSR分析中样本量及标记量对遗传多样性指标的影响
Effects of sample size and loci number on genetic diversity index in Macrobrachium rosenbergii with microsatellite markers
摘要
Abstract
As assessment of genetic structure of population is the key point of aquatic animal breeding, the selection of sample size and loci number is the first step of correct evaluation of population genetic structure. Here, we solved the problem from an analysis of genetic diversity of three breeding groups of Macrobrachium rosenbergii at 60 polymorphic microsatellite loci. The results show that selection of different sample sizes and loci numbers both had a great effect on the genetic diversity index. The sample size was positively correlated with the average number of alleles and the average effective number of alleles, and the sample size was moderate and highly correlated with heterozygosity and Nei′s genetic diversity index, respectively. Only when the sample size exceeds 30, they tend to be stable. When we select the loci, we found the different polymorphism information contents also directly affect the accuracy of the genetic parameters. When the loci number was 5–25, there was a significant difference in the genetic diversity index(P<0.05). When the loci number was more than 25, the genetic parameters changed little(P>0.05). So, we suggested that when microsatellite markers were used to estimate genetic diversity of population as far as possible to choose high polymorphism information content and we can′t consider sample gender, meanwhile the minimum loci number and sample size are needed to exceed 25 and 30, respectively.关键词
罗氏沼虾/遗传多样性/微卫星/样本量Key words
Macrobrachium rosenbergii/genetic diversity/microsatellite DNA marker/sample size分类
生物科学引用本文复制引用
戴习林,刘洁,李晶晶,江宗冰,明磊,蒋飞..罗氏沼虾种群SSR分析中样本量及标记量对遗传多样性指标的影响[J].水产学报,2017,41(7):1083-1095,13.基金项目
上海市现代农业产业技术体系(虾类)专项[沪农科产字(2015)第5号] Agriculture Research System of Shanghai (201505) （虾类）
