水产学报2017，Vol.41Issue(7)：1126-1132,7.DOI:10.11964/jfc.20160410349
锌胁迫对海洋小球藻生长和金属硫蛋白诱导的影响
Growth exhibition and metallothioneins induction of Chlorella sp. exposed to different zinc salts
摘要
Abstract
The exhibition of growth and the induction of metallothioneins of Chlorella sp. exposed to different kinds of zinc salts were investigated. The biomass, heat-stable proteins and Zn-MTs were measured when the Chlorella sp. were exposed to four kinds of Zn2+ (including zinc chloride, zinc acetate, zinc gluconate, zinc citrate) at the concentrations of 5, 10, 20, 50 and 100 μmol/L. Results showed that the growth of Chlorella sp. was not significantly inhibited when the zinc concentrations were less than 5 and 10 μmol/L for zinc citrate and zinc chloride, respectively. But the growth was significantly inhibited at the concentrations of 5 μmol/L for each of zinc acetate and zinc gluconate, and the algal biomass rapidly decreased with the increases of Zn2+ concentrations for the two zinc salts. The results also showed that the amounts of heat-stable proteins significantly increased in Chlorella sp. exposed to the four kinds of zinc salts compared with that of the control group (without adding any zinc salt), and the largest amount of heat-stable proteins was obtained when the algae were exposed to zinc gluconate at 50 μmol/L for 3 days, producing 34.5 mg of heat-stable proteins per gram of wet algae mud, which was 3.3 times higher than that of the control group. And the largest amount of Zn-MTs was induced under the inducing condition.关键词
海洋小球藻/锌胁迫/葡萄糖酸锌/生物量/金属硫蛋白Key words
Chlorella sp./zinc stress/zinc gluconate/biomass/metallothioneins分类
农业科技引用本文复制引用
彭爱红,高爽,陈俊,庄梅珍,王凡,陈晓梅,林郑忠,黄志勇..锌胁迫对海洋小球藻生长和金属硫蛋白诱导的影响[J].水产学报,2017,41(7):1126-1132,7.基金项目
福建省自然科学基金(2015J01615) （2015J01615）
厦门市海洋经济发展专项(14GZP67NF31) （14GZP67NF31）
集美大学创新团队基金(2010A007)Natural Science Foundation of Fujian Province of China (2015J01615) （2010A007）
Special Project of Xiamen Marine Economy Development of China (14GZP67NF31) （14GZP67NF31）
Foundation for Innovative Research Team of Jimei University (2010A007) （2010A007）
