水产学报2017，Vol.41Issue(7)：1148-1157,10.DOI:10.11964/jfc.20160610460
尼罗罗非鱼表皮和鳃黏膜共生菌结构特征及其与鱼体健康状况相关关系
The commensal microbiota structure of Nile tilapia (Oreochromis niloticus) skin and gill surfaces and preliminary study of their implications on tilapia health status
摘要
Abstract
Fish mucosa and their commensal microbiota serve as the first biological bulwark against invading pathogens. The purpose of this study is to study the implications on tilapia health status of the associated commensal microorganisms. Illumina sequencing was used to study the difference between the microbiota of mucosal surfaces from healthy tilapia, live and dying tilapia after Streptococcus agalactiae infection. The results showed that there were several genera dominated on tilapia skin and gill surfaces, i.e.,Truepera,Thiobacillus, Arcobacter,Marinomonas, and Vibrio. There was no significant difference between the skin and gill microbiota of the live tilapia after Streptococcus agalactiae infection and those of the initial tilapia sample. A decreased bacterial diversity was shown in the skin and gill microbiota of the dying tilapia after the pathogen infection, and the genera of Arcobacter,Pseudoalteromonas,Marinomonas,Pseudomonas and Vibrio were decreased, while the genus of Streptococcus dominated in the skin and gill microbiota of the dying tilapia after the pathogen infection, with the relative abundance of 55.30%±1.24%. The results suggested that commensal microbiota of tilapia skin and gill surface could be related to fish health.关键词
尼罗罗非鱼/皮肤/鳃/共生菌/高通量测序技术Key words
Oreochromis niloticus/skin/gill/commensal microbiota/high-throughput sequencing分类
农业科技引用本文复制引用
王淼,卢迈新,衣萌萌,曹建萌,高风英..尼罗罗非鱼表皮和鳃黏膜共生菌结构特征及其与鱼体健康状况相关关系[J].水产学报,2017,41(7):1148-1157,10.基金项目
中国水产科学研究院中央级公益性科研院所基本科研业务费专项(2015A02XK02 （）
2016HY-ZD1403) （）
广东省海洋渔业科技与产业发展专项(A201401B04) （A201401B04）
现代农业产业技术体系专项(CARS-49) Central Public-interest Scientific Institution Basal Research Fund, Chinese Academy of Fishery Science (2015A02XK02 （CARS-49）
2016HY-ZD1403) （）
Administration of Ocean and Fisheries of Guangdong Province (A201401B04) （A201401B04）
China Agriculture Research System (CARS-49) （CARS-49）
