四川医学2017，Vol.38Issue(7)：749-752,4.DOI:10.16252/j.cnki.issn1004-0501-2017.07.009
螺内酯对大鼠心肌梗死后梗死周边区超极化活化环核苷酸门控通道表达的影响
Effects of Spironolactone on HCN Expression in Infarcted Border Zone after Myocardial Infarction in Rats
余华东1
作者信息
- 1. 隆昌县人民医院心血管内科,四川 隆昌 642150
- 折叠
摘要
Abstract
Objective To investigate the changes in mRNA and protein expression of HCN2 and HCN4 in infarcted border zone after acute myocardial infarction(AMI)in rats and to discuss effects of Spironolactone on HCN2 and HCN4 expression in infarcted border zone after myocardial infarction.Methods Rat myocardial infarction model was established by ligaturing the left anterior descending coronary artery after thoracotomy.24 hours after AMI,12 survival rats were randomly divided into myocardial infarction group and Spironolactone group.6 rats in sham-operation group were only threaded through the left anterior descending coronary artery but notligatured.One week after AMI,myocardial tissue of infarcted border zone in AMI rats was sampled and in sham-operation group,corresponding parts of myocardial tissue were also sampled.mRNA level of HCN2 and HCN4 was determined by fluorescent quantitative PCR and protein level of HCN2 and HCN4 was determined by Western Blotting.Results mRNA and protein level of HCN2 and HCN4 in Myocardial infarction group and Spironolactone group was higher than sham-operation group,the difference being statistically significant(P<0.05),and mRNA and protein level of HCN2 and HCN4 in Spironolactone group was lower than myocardial infarction group,the difference being statistically significant(P<0.05).Conclusion The expression of HCN2 and HCN4 channel protein in infarcted border zone raised significantly one week after AMI,but Spironolactone can significantly decrease the expression of HCN2 and HCN4 channel protein in infarcted border zone after AMI.关键词
心肌梗死/HCN2/HCN4/螺内酯Key words
myocardial infarction/hcn2/hcn4/spironolactone分类
医药卫生引用本文复制引用
余华东..螺内酯对大鼠心肌梗死后梗死周边区超极化活化环核苷酸门控通道表达的影响[J].四川医学,2017,38(7):749-752,4.
