肿瘤预防与治疗2017，Vol.30Issue(3)：158-163,6.DOI:10.3969/j.issn.1674-0904.2017.03.002
SOX4抑制凋亡介导食管癌顺铂耐药的实验研究
Experimental Study of SOX4 Mediated Cisplatin Resistance in Esophagus Cancer by Inhibiting Apoptosis
摘要
Abstract
Objective: To explore the relationship between cisplatin resistance and the expression of SOX4,and related drug resistance mechanisms in esophagus cancer.Methods: The cisplatin resistance cell line KYSE30/DDP was established in culture by a program of treating KYSE30 parent cells in culture with increasing concentration of cisplatin.The apoptosis of KYSE30 and KYSE30/DDP cells were determined by flow cytometry.Western blot detected the expression of SOX4 and apoptosis related indicators in KYSE30 and KYSE30/DDP cells.The influence of drug sensitively by knockdown the expression of SOX4 in KYES30 and KYSE30/DDP cell was measured by CCK8 assay.The change of apoptosis and related indicators in KYSE30/DDP cell after knockdown the expression of SOX4 was evaluated by western blot and flow cytometry.Results: CCK8 assay showedthe cisplatin resistance cell line KYSE30/DDP was successfully built,and the resistance index was 4.1.The apoptosis of KYSE30/DDP cell was significantly decreased compared with KYSE30 parent cell(P<0.05).Western blot indicated the expression of SOX4 are higher in KYSE30/DDP cell compared to its parent cell.And the expression of apoptosis related indicators lower in KYSE30/DDP cell.Knock down the expression of SOX4 in KYSE30/DDP cellfacilitated thecisplatin sensitivity andpromoted cell apoptosis(P<0.05),with the increase expression of apoptosis related indicators.Conclusion: SOX4 was high-expression in cisplatin resistance cell KYSE30/DDP,and mediated cisplatin resistance by inhibiting apoptosis.关键词
食管癌/顺铂/SOX4/耐药Key words
Esophagus Cancer/SOX4/Apoptosis/Cisplatin Resistance分类
医药卫生引用本文复制引用
符立平,顾春荣,熊佳时,孙元珏,吴敬波..SOX4抑制凋亡介导食管癌顺铂耐药的实验研究[J].肿瘤预防与治疗,2017,30(3):158-163,6.基金项目
泸州市政府-西南医科大学联合项目(编号:2013LZLY-J40) （编号:2013LZLY-J40）
