肿瘤预防与治疗2017，Vol.30Issue(3)：164-167,181,5.DOI:10.3969/j.issn.1674-0904.2017.03.003
热消融不全后残存肝癌细胞HepG2生物学特性变化的实验研究
Characteristics of Residual Hepatocellular Carcinoma Cells Following Insufficient Thermo-ablation Therapy: An in Vitro Study
摘要
Abstract
Objective: To investigate the characteristics of residual HepG2 cells following insufficient thermo-ablation treatment.Methods: The HepG2 celles were heated to 47℃ for 5min,7.5min,10min respectively,and the normal HepG2 cells were control.All cells were observed under microscope and MTS assay was used to determine residual cells proliferation at 24h,48h and 72h.Transwell assay was used to detect the migration of the residual cells;(4) The ability of clone was observed by cell culture for 10 days.Results: The results showed that with the increasing heating time,the apotosis cells increased.The proliferation of residual cells enhanced within 24h,and decreased in 48-72h group.The ability of migration of residual cells enhanced with the increased heating time.The ability of clone was reduced after heat treatment.Conclusion: The characteristics of residual HepG2 cells were changed after insufficient thermo-ablation treatment.The slope proliferation was enhanced and declined along with the heating time extension.The ability of migration was unregulated,while the ability of clone was reduced.关键词
热消融/肝癌/复发/转移Key words
Thermo-ablation/Hepatocellular carcinoma/Recurrence/Metastasis分类
医药卫生引用本文复制引用
秦威,阮镜良,梁铭,许晓琳,田晶,杨海云,罗葆明..热消融不全后残存肝癌细胞HepG2生物学特性变化的实验研究[J].肿瘤预防与治疗,2017,30(3):164-167,181,5.基金项目
国家自然科学基金面上项目(编号:81671 704) （编号:81671 704）
广东省自然基金自由申请项目(编号:2016A0303 13306) （编号:2016A0303 13306）
