山东中医杂志2017，Vol.36Issue(8)：710-713,4.
正交实验法优选发酵蜈蚣粉中水溶性总蛋白提取工艺及其抗肿瘤效果
Optimization of Extraction Process for Total Water Soluble Proteins in Fermented Centipede Powders by Orthogonal Test
摘要
Abstract
Objective:To optimize extraction process for total water soluble proteins in fermented centipede powders. Method:With the water soluble proteins as the index,the orthogonal experiment design method of L9 (34) was used to evaluate the influence by extraction time,pH value,and material–liquid proportions on content of the water soluble total proteins of centipede powder ,based on the study for single factors ,and the optimized extraction process was determined. Results:The optimized extraction process for water-soluble total protein from the fermented centipede powder was as follows:adding 20-fold distilled water with pH value of 9 and under ultrasonic extraction for 150 min;the water soluble extractions of the fermented centipede pow-der had a good inhibitory effect on mammary gland MCF-7 cells. Conclusion:In this experiment,the opti-mized extraction process of total protein from fermented centipede powder was convenient and feasible ,and can accurately reflect the total protein content with certain anti-tumor effect.关键词
发酵蜈蚣粉/总蛋白/MTT法/乳腺癌MCF-7细胞株/肺腺癌A549细胞株/细胞抑制率Key words
fermented centipede powders/total protein/MTT/breast cancer MCF-7 cell line/lung adenocarci-noma A549 cell line/cell inhibition rate分类
医药卫生引用本文复制引用
史磊,刘春雨,张盼盼,王彦多,王颖,王集会..正交实验法优选发酵蜈蚣粉中水溶性总蛋白提取工艺及其抗肿瘤效果[J].山东中医杂志,2017,36(8):710-713,4.基金项目
山东中医药大学大学生研究训练(SRT)计划资助项目(校编号:2015308,国家编号:201610441069) （SRT）
