山东中医杂志2017，Vol.36Issue(8)：714-716,724,4.
菊芋蛋白的提取及其降血糖作用研究
Extraction of Helianthus Tuberosus Protein and Its Hypoglycemic Activity
杨稳 1刘飞 1刘文静 1周长征1
作者信息
- 1. 山东中医药大学,山东 济南 250355
- 折叠
摘要
Abstract
Objective:To compare the extraction rate of two extraction methods for Helianthus tuberosus pro-tein,and to evaluate its antidiabetic effect. Methods Acetone precipitation and ammonium sulfate salting-out methods were applied for protein extraction ,and protein content was applied as the index to optimize the extraction method,and the hypoglycemic activity was evaluated in the mouse. Results:The protein extraction rate by acetone precipitation was 11.30%,while that by ammonium sulfate salting-out method was 10.80%. The high dose of Helianthus tuberosus protein showed obvious hypoglycemic activity. Conclusion:Acetone sedimentation method is of high extraction rate ,and Helianthus tuberosus protein is of hypoglycemic activity.关键词
菊芋蛋白/丙酮沉淀法/硫酸铵盐析法/蛋白质电泳/紫外测含量/降血糖Key words
Helianthus tuberosus protein/acetone precipitation/ammonium sulfate salting-out method/protein electrophoresis/ultraviolet spectrophotometry/hypoglycemic activity分类
医药卫生引用本文复制引用
杨稳,刘飞,刘文静,周长征..菊芋蛋白的提取及其降血糖作用研究[J].山东中医杂志,2017,36(8):714-716,724,4.
