山东医药2017，Vol.57Issue(25)：5-7,3.DOI:10.3969/j.issn.1002-266X.2017.25.002
Piscidinol A对人肝癌HepG-2细胞增殖、凋亡的影响
Effects of piscidinol A on proliferation and apoptosis of human liver cancer HepG-2 cells
摘要
Abstract
Objective To investigate the effects of piscidinol A on apoptosis and proliferation of human liver cancer cell line HepG-2 and the mechanism. Methods HepG-2 cells in the logarithmic phase were cultivated for 24h and then were divided into the drug group and the control group after pancreatic enzyme digesting. The drug group was treated by dif-ferent concentrations of piscidinol A (6. 25,12. 5,25,50,and 100 mg/ L)and the control group was treated by the same volume RPMI1640 culture which was contained 10% fetal bovine serum. MTT assay was applied to detect the proliferation inhibitory rate at 12,24,and 48 h. IC 50 of piscidinol A was calculated,and the morphology of apoptotic cells was observed with Hoechst 33258 fluorescence staining. Flow cytometry was applied to detect apoptosis rate of HepG-2 cell by using An-nexin Ⅴ-FITC/ PI double staining. Western blotting was used to detect the B-cell lymphoma/ leukemia-2 associated x (Bax)expression in cells. Results In the drug group,piscidinol A had remarkable proliferation inhibition effect on HepG-2 cells in a dose and time-dependent manner,its IC 50 was 24. 90 mg/ L at 24 h. Most of cells presented the typical morphological changes of apoptosis under the fluorescent microscope and more effect occurred with the increasing concentra-tion. After the cells were treated by different concentrations of piscidinol A (6. 25,12. 5,25,50,100 mg/ L)for 24 h, the apoptosis rates were 11. 83%,17. 49%,21. 72%,34. 17%,and 48. 51%,respectively in the drug group,versus 7. 23% in the control group (all P < 0. 05);the apoptotic protein expression of Bax was 0. 29,0. 33,0. 35,0. 39,and 0. 43 μg/ μL,respectively in the drug group,versus 0. 24 μg/ μL in the control group. The apoptosis rate and Bax protein expression increased with the increasing concentrations of piscidinol A in the drug group,and the difference was statistically significant as compared with that of the control group (all P < 0. 05). Conclusion Piscidinol A inhibits the proliferation and induces apoptosis of human liver cancer HepG-2 cells.关键词
肝肿瘤/HepG-2细胞/匹西狄醇A/细胞增殖/细胞凋亡/B细胞淋巴瘤/白血病基因伴随蛋白xKey words
hepatoma/HepG-2 cells/piscidinol A/cells proliferation/apoptosis/B-cell lymphoma/ leukemia-2 as-sociated x分类
医药卫生引用本文复制引用
肖雪琴,张敏鸿,刘海,杨建琼..Piscidinol A对人肝癌HepG-2细胞增殖、凋亡的影响[J].山东医药,2017,57(25):5-7,3.基金项目
江西省卫生厅中医药科研基金项目(2014A114) （2014A114）
赣州市指导性科技计划项目(GZ2014ZSF125) （GZ2014ZSF125）
江西省教育厅青年科学基金项目( GJJ150939) （ GJJ150939）
江西省科技厅自然科学基金计划项目(2015ZBAB205005,20161BAB215220). （2015ZBAB205005,20161BAB215220）
